维生素C对光化学法诱导皮肤光损伤的光保护作用:OCT定量研究

Quantitative Analysis on PUVA-induced Skin Lesions and the Photoprotective Effect Induced by Vitamin C Using Optical Coherence Tomography

8-甲氧补骨脂素(8-MOP)联合UVA辐射(即光化学疗法PUVA)因诱导皮肤光损伤的副作用,常被用于实验动物皮肤光损伤模型的构建。为研究维生素C(Vc)对皮肤光损伤的保护效应,本研究在Balb/c小鼠背部皮肤涂抹0.1%8-MOP溶液1h后进行UVA辐照(10 J/cn2)。然后分别涂抹7.5%、15%和30%浓度的Vc溶液的进行光保护治疗,利用光学相干层析成像分析皮肤厚度和光信号衰减的变化,从而评估Vc对皮肤的光保护作用。结果显示,相对于溶媒组,Vc治疗组皮肤厚度减小,光衰减系数增加,其中15%效果尤为明显结果表明,局部涂抹Vc溶液具有一定的光损伤保护效应。

Psoralen plus ultraviolet A radiation （PUVA） therapy is a very important clinical treatment of skin diseases such as vitiligo and psoriasis, but associated with an increased risk of skin photodamages. To study the photoprotective effect of vitamin C（Vc） to PUVA induced photodamaged skin, 7.5% , 15% ,30% Vc solutions were applied to PUVA induced photodamaged skin of the Balb/e mice daily for 3 days. OCT was introduced to measure the antioxidant protec- tion factor by analysis of the changes in optical parameters. The results showed that Vc treatment group had a decrease in shin thickness and an increase in attenuation coefficient. In conclusion, the vitamin C treatment, especially 15% Ve so- lution treatment, may promote the repair process of photodamage.