放射损伤对小鼠外周血T淋巴细胞亚群的影响

Influence of radiotherapy lession on T-Lymphocyte subsets in peripheral blood of the mice

目的:分析放射治疗前、后小鼠外周血T淋巴细胞亚群的变化,探讨放射治疗所致的免疫系统损伤在细胞学和分子水平上的机制。方法:流式细胞术分别检测不同放射剂量昆明小鼠的外周血中CD3+、CD4+、CD8+及CD4+CD25high调节性T细胞比例。结果:放射治疗组外周血CD3+、CD4、CD8+T细胞比例低于未放射治疗组(P<0.05);CD4+CD25high调节性T细胞比例高于未放射治疗组(P>0.05)。结论:放射治疗可造成小鼠T淋巴细胞亚群抑制,有可能成为肿瘤患者有效的免疫监测指标。

Objective： To analysize the changes in T-Lymphocyte Subsets in peripheral blood of the mice,and investigate the cellular and molecular mechanism of immune system injury induced by irradiation.Methods： The peripheral blood was collected from different radiotherapy dose of the mice.The changes of T-lymphocyte subsets CD3^＋,CD4^＋,CD8^＋ and CD4＋CD25highregulatory T cells were analyzed by flow cytometry.Results： TheT lymphocyte subsets CD3＋,CD4＋,CD8＋ have distinctly reduced after irradiation（P〈0.05）;CD4＋CD25highregulatory T cells shows marked increase compared with that the control group（P〈0.05）.Conclusion： The radiotherapy can inhibit T lymphocyte subsets in mice which can be a useful index for monitoring the immunity function.