NF-κB在心肌梗死中表达量的变化及其意义

Expression Variation of NF-κB in Myocardial Infarction

目的:为了研究NF-κB p65表达量在心肌梗死中的变化。方法:对大鼠进行心肌梗死造模,而后运用半定量PCR和Western blot技术分别在mRNA和蛋白水平上检测梗死心肌组织中p65表达量的变化。结果:造模成功,且在手术组中,p65在mRNA和蛋白水平上的表达量明显高于假手术组和正常组(P<0.05)。结论:NF-κB p65的表达量与心肌梗死有密切关系。

Objective：To study the expression variation of NF-κB p65 in myocardial infarction.Methods： Myocardial infarction model in rats was made.The expression of p65 in mRNA and protein level was detected by PCR and Western blot.Results： The model was made successfully.And in the operation group,the expression of p65 in mRNA and protein level was higher than the sham group and normal group（P〈0.05）.Conclusion： NF-κB p65 expression is closely related to myocardial infarction.