己酮可可碱对大鼠肝脏缺血再灌注损伤的影响

Effect of Pentoxifylline on rat hepatic ischemia/reperfusion injury

目的:探讨TNF-α抑制剂己酮可可碱对大鼠肝脏缺血再灌注损伤(HIRI)的影响。方法:持续阻断肝固有动脉1 h后再灌注1 h,建立SD大鼠HIRI模型。24只雄性SD大鼠随机分成4组:生理盐水腹腔预处理假手术组(A)、己酮可可碱腹腔预处理假手术组(B)、生理盐水腹腔预处理HIRI组(C)、己酮可可碱腹腔预处理HIRI组(D)。检测各组血清ALT和肝组织HE染色、原位凋亡(TUNEL)和Ki-67。结果:D组与C组相比,ALT显著降低(P<0.05),组织病理损伤程度明显减轻,TUNEL染色阳性细胞、Ki-67阳性细胞显著减少(P<0.05);而D组与B组相比,血清ALT升高(P<0.05),组织轻度损伤,Ki-67阳性细胞数及TUNEL阳性细胞数均无明显差异。与A组相比,B组各项指标变化不明显。结论:HIRI早期TNF-α抑制剂可抑制肝细胞凋亡和再生,从而减轻HIRI。

Objective： To investigate the effect of Pentoxifylline, a TNF-α inhibitor, on hepatic ischemia / reperfusion injury （HIRI） in rats. Methods： HIRI model in Sprague-Dawley （SD） rats was established by clamping the hepatic artery for 1 h followed by lh reperfusing. Twenty-four male SD rats were randomly divided into 4 groups： （A） NS preconditioning by intra-peritoneal injection without HIRI. （B） Pentoxifylline preconditioning by intra-peritoneal injection without IRI. （C） NS preconditioning by intra-peritoneal injection 1 h before HIRI. （D） Pentoxifylline preconditioning by intra-peritoneal injection lh before IRI. Hepatic histomorphology analysis was conducted. Levels of serum ALT, Ki-67 expression and apoptosis in hepatic tissue were investigated. Results： Comparing with group C, serum ALT level in group D decreased significantly （P 〈0.05 ）. The tissue injury of group D was less severe, with less TUNEL positive ceils （ P 〈 0.05 ） and more Ki-67 positive cells （ P 〈 0. 05 ）, Comparing with group B, serum ALT level in group D increased slightly （P 〈 0. 05 ）, with a milder tissue injury. But there was no significant difference in Ki-67 positive cells and TUNEL positive cells among these groups. There is no significant difference in all observed paremeters between group A and B. Conclusion： In the early phase of liver ischemia-reperfusion, Pentoxifylline may simultaneously suppress apoptosis and regeneration of hepatic cells, leading to relief of hepatic ischemia/reperfusion injury.