血清高迁移率族蛋白1与急性胰腺炎严重程度相关性研究

The relationship between serum high mobility group box chromosomal protein-1 levels and the severity of acute pqncreatitis

目的观察急性胰腺炎（AP）患者血清高迁移率族蛋白1（high mobility group box chromosomal protein-1，HMGBl）的水平，探讨HMGBl水平与AP严重程度的相关性。方法收集62例患者人院即刻及入院后24、8h血标本，应用ELISA方法检测血清HMGBl水平，并分析其与AP病情严重程度的相关性。以20例健康成年人作为对照组。结果62例AP患者人院即刻及人院后24、48h血清HMGBl水平分别为（8．05±1．60）、（8．04±1．39）、（8．25±1．56）ng／ml，均显著高于对照组的（2．20±0．57）mg／ml（P值均〈0．01）。其中重症急性胰腺炎（SAP）35例，轻症急性胰腺炎（MAP）27例。SAP组患者血清HMGBl水平分别为（7．994-1．69）、（8．12±1．40）、（8．134±1．34）ng／ml；MAP组患者为（8．12±1．52）、（7．92±1．40）、（8．394±1．81）ng／ml。两组间差异无统计学意义。结论AP患者的血清HMGBl水平显著高于正常人群，但其与AP的严重程度无平行关系。

Objective To investigate the high mobility group box chromosomal protein-1 （HMGB1） levels in patients with acute pancreatitis （AP）; and to study the relationship between the serum level of HMGB1 and the severity of AP. Methods The patients＇ serum HMGB1 concentrations were determined right after admission, 24, 48 hour after admission. The levels of HMGB1 were measured by ELASA kit and its relationship with the severity of AP was analyzed. 20 healthy adults were treated as the control group. Results At the time of admission, and 24, 48 hours after admission, the serum HMGB1 levels in AP patients were （8.05 ± 1.60）, （ 8.04 ± 1.39 ）, （ 8.25 ±1.56） ng/ml, respectively, which were significantly higher than that in the healthy control [ （2.20 ± 0.57）ng/ml, P 〈 0. 01 1. There were 35 patients with severe acute pancreatitis （SAP） and 27 patients with mild acute pancreatitis （MAP）. The HMBG1 levels in patients with SAP were （7.99 ±1. 69）, （8.12 ±1. 40）, （8.13±1. 34） ng/ml, and they were （8.12 ± 1.52）, （7.92 ± 1.40）, （8.39 ± 1.81）ng/ml in patients with MAP, and the difference between the two groups was not statistically significant. Conclusions The serum HMGB1 level in AP patients was significantly higher than that in healthy controls, but it was not related with the severity of AP.