RUNX3、CyclinDl在胰腺癌组织中的表达及临床意义

Expressions and significances of RUNX3 and CyclinDl in pancreatic carcinoma

目的检测RUNX3、CyclinDl蛋白在胰腺癌组织中的表达，分析其临床意义。方法采用免疫组化sP法检测47例胰腺癌、18例胰腺囊腺瘤及12例正常胰腺组织中RUNX3和CyclinDl蛋白的表达，分析它们与临床病理参数间的关系。结果胰腺癌、胰腺囊腺瘤和正常胰腺组织的RUNX3蛋白阳性表达率分别为57．4％（27／47）、94．4％（17／18）、100％（12／12）；CyclinDl蛋白阳性表达率分别为72．3％（34／47）、44．4％（8／18）、8．3％（1／12）。胰腺癌RUNX3阳性表达与患者性别、年龄无关，与胰腺癌的临床分期、淋巴结转移、分化程度呈负相关（P〈0．05）。CyclinDl阳性表达与患者性别、年龄无关，与胰腺癌临床分期、淋巴结转移、分化程度呈正相关（P〈0．05）。胰腺癌组织RUNX3与CyclinDl的表达呈负相关（r=-0．375，P=0．009）。结论胰腺癌组织中RUNX3呈低表达，Cyclin D1呈过表达，它们均与胰腺癌的发生、发展有关。

Objective To investigate the expressions of RUNX3 and CyclinD1 in pancreatic carcinoma and their significance. Methods Expressions of RUNX3, CyclinD1 in 47 cases with pancreatic carcinoma, 18 cases with cystadenoma of pancreas and 12 normal pancreas cases were detected by immunohistochemistry, and the relationship between their expressions and clinicopathological parameters was analyzed. Results The positive expression rates of RUNX3 in pancreatic carcinoma, cystadenoma of pancreas, normal pancreas cases were 57.4% （ 27/47 ）, 94.4% （ 17/18 ）, 100% （ 12/12 ） ; the positive expression rates of CyclinD1 in pancreatic carcinoma, cystadenoma of pancreas, normal pancreas cases were 72.3% （ 34/47 ）, 44.4% （8/18）, 8.3% （1/12）. RUNX3 expression was not related to the age and sex, but it was negatively associated with clinical staging, lymph node metastasis, the differentiation degree （P 〈0.05 ）. CyclinD1 expression was not related to the age and sex, but it was positively associated with clinical staging, lymph node metastasis, the differentiation degree （P 〈0.05 ）. The expression of RUNX3 and CyclinD1 was negatively associated （r = - 0.375, P - 0.009）. Conclusions The expression of RUNX3 is decreased in pancreatic carcinoma. The expression of CyclinD1 is increased in pancreatic carcinoma. They may play an important role in the carcinogenesis and progression of pancreatic carcinoma.