蜂毒素对胃癌SGC-7901细胞裸鼠移植瘤的抑制作用及其部分机制

Study of melittin on tumor inhibition and partial mechanism of human gastric cancer SGC-7901 cell xenografts in nude mice

目的研究蜂毒素对人胃癌SGC-7901细胞裸鼠移植瘤的抑制作用,并初步探讨其作用机制。方法建立人胃癌SGC-7901细胞裸鼠移植瘤模型,采用抑瘤率指标评价蜂毒素对荷瘤小鼠的抑瘤作用,采用脏器指数、中性红法测定腹腔巨噬细胞吞噬功能,改良的四甲基偶氮唑盐(MTT)比色法检测自然杀伤(NK)细胞活性,ELISA法测定血清白介素2(IL-2)的含量,观察机体免疫变化情况。结果蜂毒素(2、4mg/kg)能抑制荷瘤小鼠的肿瘤生长,提高脾脏指数,增强腹腔巨噬细胞吞噬功能和NK细胞的活性,蜂毒素(4 mg/kg)能增加血清中IL-2的含量。结论蜂毒素对荷瘤小鼠具有较强的抑瘤作用,可能与其提高机体免疫功能有关。

Objective To explore the effects and mechanism of melittin on tumor growth of human gastric cancer SGC-7901 cell xenografts in nude mice.Methods The xenografts derived from SGC-7901 cells were established in nude mice.The effect of melittin on tumor-bearing mice was evaluated by inhibition rate.Phagocytosis of peritoneal macrophage was measured by neutral red method.NK cell activity was detected by improved MTT assay.Concent of interleukin-2（IL-2） in serum was determined by ELISA method.The changes of immunity of tumor-bearing mice were observed by above-mentioned and organ index.Results Melittin（2,4 mg/kg） could inhibit the tumor growth,while improve the index of spleen,enhance phagocytosis of peritoneal macrophage and NK cell activity,and melittin（4 mg/kg） could increase IL-2 level in serum.Conclusion Melittin has significant inhibition on tumor-bearing mice,which is related to reinforcing the immunity of mice.