蜂毒素全氟碳纳米囊的制备及评价

Preparation and evaluation of melittin perfluorocarbon nanocapsules

目的制备蜂毒素全氟碳纳米囊,考察其外观特征、粒径、Zeta电位、电镜形态、包封率及稳定性。方法以全氟碳为纳米核心,通过高压均质法制备蜂毒素纳米囊。采用透射电镜和激光粒度分析仪检测纳米颗粒形态、粒径及Zeta电位,双波长考马斯亮蓝法测定纳米颗粒载药包封率。将制备的蜂毒素全氟碳纳米囊乳液置于4℃下密封贮藏30 d,以纳米囊的粒径和包封率的变化作为指标,考察其稳定性。结果蜂毒素全氟碳纳米囊的平均粒径为84.41 nm,多分散系数为0.115,Zeta电位为-52.1 mV。采用双波长考马斯亮蓝法测定蜂毒素全氟碳纳米囊包封率,在吸收度及线性均良好的情况下,考马斯亮蓝法检测游离蜂毒素含量的检测范围为0.5～25 mg/L,该范围内线性良好,相关系数为0.9993,蜂毒素药物的包封率为(86.31±0.76)%(n=3),RSD<1%,稳定性良好。结论采用高压均质法制备蜂毒素全氟碳纳米囊的方法可行,经粒径、形态、包封率方面的考察,结果良好。

Objective To prepare melittin perfluorocarbon（PFC） nanocapsules（NPs） and investigate the particle size,Zeta potential,morphology of electron microscopy（EM）,entrapment efficiency and stability.Methods Melittin loaded within PFC NPs were constructed by high pressure homogeneous processing method.The morphology and size of NPs were detected by transmission electron microscopy（TEM） and laser particle size analyzer.Drug entrapment efficiency was assessed by two-colorimetric Coomassie brilliant blue.The stability of melittin PFC NPs was investigated by evaluating the change of its average particle size and entrapment efficiency during storage at 4℃for 30 days.Results The average particle size of melittin PFC NPs was 84.41 nm,polydispersity was 0.115,Zeta potential was-52.1 mV.The linear range of melittin was 0.5～25 mg/L（r=0.999 3）.Entrapment efficiency of three batches of melittin PFC NPs was（86.31±0.76）%（n=3） with RSD less than 1%.Conclusion It is feasible that melittin loaded within PFC NPs can be constructed by high pressure homogeneous processing method.The investigation results are good by the particle size,morphology of EM and entrapment efficiency.