胰腺癌中Tenascin-C蛋白和微血管密度的检测及其临床意义

Detections of Tenascin-C and microvessel density in pancreatic cancer and their clinical significance

目的探讨Tenascin-C与微血管密度(MVD)在胰腺癌组织中的表达及其与胰腺癌临床病理学特征间的关系。方法收集49例胰腺癌标本及10例正常胰腺组织,采用免疫组化SP法检测Tenascin-C与MVD表达,并比较其与临床病理特征间的关系。结果①胰腺癌组织中Tenascin-C阳性率及MVD明显高于正常胰腺组织(P<0.05);②胰腺癌中淋巴结转移组Tenascin-C和MVD明显高于无淋巴结转移组,其表达与患者的年龄、性别、肿瘤部位均无关;③Tena-scin-C与MVD的表达在胰腺癌中呈正相关(r=0.367,P<0.01)。结论 Tenascin-C与MVD可能存在协同作用,两者联合检测对判断胰腺癌的血管生成及浸润转移有重要价值。

Objective To investigate the expressions of Tenascin-C and microvessel density （MVD） in pancreatic cancer and to study the relationship with tumor angiogenesis and clinical significance. Methods The expressions of Tenascin-C and MVD were examined in 49 cases of pancreatic cancer and 10 cases of normal pancreatic tissue by SP immunohistochemical technique. Results （1） The positive of Tenascin-C was 69.4% in pancreatic cancer and it was not detected in normal tissues, while MVD showed extensive expression in pancreatic carcinoma tissues, but limited in normal bile duct ,which showed significant difference（ P 〈 0. 05 ）. （2） In pancreatic carcinoma, Tenascin- C and MVD in the group with lymph node metastasis was significantly higher than that in the group without lymph node metastasis. （3） The positive expression of Tenascin-C was associated with MVD （ r = 0. 367, P 〈 0. 01 ）. Conclusion Tenascin-C was overexpressed in pancreatic cancer compared with negative expression in normal tissues, which suggested Tenascin-C might be biological marker in pancreatic cancer. The levels of Tenascin-C and MVD are useful molecular markers for evaluating angiogenesis and metastasis.