淋巴管癌栓检测及与乳腺癌临床病理特征的关系

Detection of lymph vessel tumor emboli in breast cancer and its relation with clinicopathologic characteristics

目的比较淋巴管内皮标记物D2-40和HE染色对乳腺癌淋巴管癌栓的检出率以及D2-40阳性的淋巴管癌栓与乳腺癌临床病理特征的关系。方法选取110例浸润性乳腺癌,分别进行HE染色及免疫组化D2-40、CD31、CD34、p63染色;应用SPSS17.0统计软件进行分析。结果 HE染色的淋巴管癌栓检出率为15.5%,假阳性率为2.8%,假阴性率为23.6%;而应用D2-40标记的淋巴管癌栓检出率为36.5%,两者比较差异显著(P<0.05)。淋巴管癌栓与患者的年龄、肿瘤大小、组织学分级、淋巴结转移、ER低表达具有显著的统计学意义,与PR、HER-2表达无统计学意义。结论单克隆抗体D2-40是可靠的淋巴管内皮标记物,与HE染色相比能显著提高淋巴管癌栓的检出率。

Objective To compare lymph vessel tumor emboli（LVTEs） detected by HE and immunohistochemical staining of D2-40 antibodies and to investigate whether D2-40＋ LVTE is related with the clinicopathologic characteristics.Methods A total of 110 cases of invasive breast cancer were selected in this study.Lymph vessels were evaluated by immunohistochemistry using the D2-40,CD31,CD34,p63 and HE stain,and software SPSS17.0 was used to analyse the results.Results The positive rate of LVTEs by HE staining was 15.5%,with false-positive rate of 2.8% and false-negative rate of 23.6% by HE staining.LVTEs detected rate by D2-40 was 36.5%,and thus D2-40 staining improveds the rate of LVTEs by 20.9% compared with HE stain.LVTE was positively related to age,tumor size,higher histologic grede,and node status and versely related to ER status.There was no significant correlation between D2-40＋ lymph vessel tumor emboli and PR and HER-2 expression.Conclusion D2-40 is a reliable marker of LVTE and improves the detection of LVTE compared to routine HE-staining.LVTE is related to the age,higher histologic grade,axillary node status and estrogen expression.