急性白血病患者外周血Treg细胞与Th17细胞的相关性

Relationship between Treg and Th17 cells in peripheral blood of acute leukemia patients

目的:检测急性白血病患者外周血中Treg细胞与Th17细胞的比例以及相关细胞因子如IL-17、IL-6、TGF-β的变化,分析其相关性。方法:选取兰州大学第二医院血液科急性白血病初诊患者15例,另取15名健康志愿者为对照。流式细胞术检测外周血中CD3+CD4+TIL-17+辅助性T细胞(Th17细胞)、CD4+TCD25+Foxp3+调节性T细胞(Treg细胞)占CD4+T细胞的比例,ELISA法检测血清中细胞因子IL-17、TGF-β、IL-6的水平。结果:急性白血病患者外周血中Th17细胞占CD4+T细胞的(1.39±0.24)%,高于对照组的(0.26±0.11)%(P<0.05);急性白血病患者外周血Treg细胞占CD4+T细胞的(11.58±2.17)%,高于对照组的(2.47±0.72)%(P<0.05);且Treg细胞与Th17细胞呈正相关(γ=0.37)。急性白血病患者血清中TGF-β、IL-6、IL-17的水平分别为(26.06±2.43)、(14.66±2.47)、(18.63±2.38)pg/ml,高于对照组的(13.41±1.92)、(1.44±0.29)、(10.34±1.71)pg/ml(均P<0.05)。结论:急性白血病患者外周血中Treg、Th17细胞比例升高,且两者呈正相关;急性白血病患者血清中TGF-β、IL-6、IL-17水平升高,可能影响Treg与Th17细胞的平衡。

ive： To observe the proportions of CD3＋CD4^＋TIL-17^＋ helper T cells（Th17 cells） and CD4^＋TCD25^＋Foxp3^＋ regulatory T cells（Tregs） in the peripheral blood of acute leukemia patients and the changes of related cytokines（including IL-17,TGF-β,IL-6）,and to analyze their relationship.Methods： The ratios of Treg and Th17 cells in peripheral CD4^＋T cells were examined by flow cytometry.The serum levels of IL-17,TGF-β and IL-6 were measured by ELISA.Results： The ratio of Th17 cells in CD4^＋T cells was（1.39±0.24）%,which was significantly higher than that in the control group（[0.26±0.11]%,P〈0.05）;the ratio of Treg cells in CD4^＋T cells was（11.58±2.17）%,which was significantly higher than that in the control group（[2.47±0.72]%,P〈0.05）;and Treg cells were positively related with Th17 cells.The serum levels of TGF-β,IL-6,and IL-17 in acute leukemia patients were（26.06±2.43）,（14.66±2.47）,and（18.63±2.38） pg/ml,respectively,which were significantly higher than those in the control group（[13.41±1.92],[1.44±0.29],and [10.34±1.71] pg/ml,P〈0.05）.Conclusion： The ratios of Treg and Th17 cells are increased in the peripheral blood of acute leukemia patients,and they are positively correlated with each other.The serum levels of TGF-β,IL-6,and IL-17 are also elevated,which may affect the balance between Treg cells and Th17 cells.