卡托普利对MC3T3-E1细胞增殖、分化及Ⅰ型胶原mRNA表达的影响

Effects of captopril on the proliferation,differentiation and the mRNA expression of Type Ⅰ Collagen in MC3T3-E1 cells

目的观察卡托普利对MC3T3-E1细胞增殖、分化和Ⅰ型胶原基因表达水平影响。方法在MC3T3-E1细胞中加入不同浓度的卡托普利,应用MTT法、对硝基苯磷酸盐法、RT-PCR的方法观察卡托普利对MC3T3-E1细胞的增殖、碱性磷酸酶活性和Ⅰ型胶原mRNA表达的影响。结果 10-11 mol.L-1浓度的卡托普利作用24 h能明显促进MC3T3-E1细胞增殖,作用3 d能明显促进MC3T3-E1细胞表达碱性磷酸酶。卡托普利可刺激MC3T3-E1细胞表达Ⅰ型胶原,以10-11 mol.L-1作用最强。结论卡托普利有促进MC3T3-E1细胞增殖、促进碱性磷酸酶表达和增加Ⅰ型胶原mRNA表达的作用。

Aim To investigate the effects of captopril on the proliferation, differentiation and the mRNA ex- pression of Type I Collagen （ COLL- I ） in MC3T3-E1 cells. Methods The Mq＇T method was employed to detect the cells proliferation after culture with captopril for 12 h,24 h and 36 h respectively. The cell differentiation was evaluated by detecting activities of alkaline phosphatase （ALP） using PNPP method after culture with captopril for 3 d, 5 d and 7 d respectively. Re- verse transcription-Polymerase chain reaction （RT- PCR） was used to screen the level of Type I CollagenmRNA expression after incubation with captopril for 7 d. Results Captopril accelerated cell growth with 10-11 mol · L-1 concentration after 24 h. After 7 days of stimulation with 10-11 mol· L-1 captopril, ALP activity increased significantly. Captopril up-regulated COLL-I mRNA expression at a concentration of 10-11 mol · L-1. Conclusion Captopril stimulates the dif- ferentiation, proliferation and up-regulates mRNA expression of MC3T3-E1 cells in vitr, COLL- I mRNA expression of MC333-E1 cells in vitro.