梓醇改善东莨菪碱诱导的学习记忆障碍及机制研究

Effects of catalpol on scopolamine-induced learning and memory dysfunction in mice

目的研究梓醇对由东莨菪碱所致小鼠空间学习记忆障碍的影响和机制。方法小鼠随机分为4组:正常组、模型组(东莨菪碱,2 mg.kg-1)、奥拉西坦组(阳性药物,105mg.kg-1)和梓醇治疗组(9 mg.kg-1),采用水迷宫测试小鼠空间学习记忆功能。于实验前30 min腹腔注射东莨菪碱诱导小鼠学习记忆障碍模型,记录小鼠逃避潜伏期时间。水迷宫测试毕,处死小鼠,分离大脑皮质和海马,匀浆取上清液测大脑皮质和海马中乙酰胆碱(acetylcholine,ACh)和脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)的含量。结果①各组实验前逃避潜伏期无差异;给药东莨菪碱前后差异有显著性。②组间比较,与正常组比较,东莨菪碱导致记忆障碍;与东莨菪碱组比较,阳性药物奥拉西坦和梓醇治疗组逃避潜伏期明显缩短,ACh和BDNF含量明显增加(P<0.05)。结论小鼠注射东莨菪碱成功诱导学习记忆机能障碍模型。梓醇改善东莨菪碱诱导的学习和记忆障碍,其机制可能与促进BDNF表达,增加脑ACh含量有关。

Aim To assess effects of catalpol on spatial learning and memory dysfunction induced by scopolam- ine in mice. Methods The mice were divided into 4 groups ： control group, model group （ scopolamine, 2 mg ·kg-I ） , positive group （ oxiracetam, 105 mg · kg-1 ） and catalpol group （9 mg · kg-1）. The learning and memory of mice were evalauated by Morris water maze test. 30 min before the test, drugs were given by intrap- eritoneal injection and escape latencies were recorded. In later experiments, the animals were sacrificed and the cerebral cortex and hippocampus were quickly re moved and homogenated. ACh and BDNF in mice cere- bral cortex and hippocampus were tested. Results （1） The Morris water maze test showed that there were no differences in escape latencies in all groups before giving scopolamine. But there were obvious differencesafter giving scopolamine, t, 2） Uompareu wltn me con- trol group, scopolamine induced learning and memory dysfunction. Compared with the model group,the mice in positive group and catalpol group had shorter escape latencies and the content of ACh and BDNF were in- creased obviously （ P 〈 0. 05 ）. Conclusions The mice injected with scopolamine imitate the amnesia an- imal models sucessfully. Catalpol can improve the learning and memory dysfunction induced by scopolam- ine. The mechanism may be related to the promotion of BDNF expression and the increase of the content of ACh in brain.