人鼻息肉黏膜上皮细胞3种原代体外培养方案的比较

Comparative study of three primary culture methods of human epithelial cells of nasal polyps in vitro

目的:比较人鼻息肉黏膜上皮细胞3种原代体外培养方法的效果,探讨适宜的体外培养方案。方法:对人鼻息肉黏膜上皮细胞分别以酶消化分离法、酶消化分离细胞ABC法和组织块培养法进行原代体外培养,观察细胞形态,掌握其生物学特性,并对3种方案的培养成功率和细胞生长曲线进行比较。结果:酶消化分离细胞ABC法体外培养成功率为87.5%,优于酶消化分离法(78.13%)和组织块培养法(84.38%),但三者间差异无统计学意义(P>0.05)。酶消化分离法和酶消化分离细胞ABC法的细胞生长曲线无明显差异,均高于组织块培养法。结论:酶消化分离细胞ABC法细胞增殖迅速,无其他细胞混杂,可建立稳定、可靠的人鼻黏膜上皮细胞原代培养模型,可为鼻息肉的相关研究提供良好的细胞系。

Objective：To compare three primary culture methods of human epithelial cells of nasal polyps in vitro,and explore a suitable method to be used in further study.Method：Shape and appearance of human epithelial cells of nasal polyps were observed,and success ratio and growth curve of primarily cultural were achieved by enzymatic dissociation,isolated cell ABC culture and tissue piece culture method respectively.Result：By comparing achievement ratios,the isolated cell ABC culture method（87.5%） was shown to be superior to enzymatic dissociation（78.13%） and tissue piece culture methods（83.33%）,but no statistical significance was found（P0.05）.The growth curves of two isolated cell culture methods were higher than that of tissue piece culture.Conclusion：The isolated cell ABC culture model is more suitable for primary culture due to its faster proliferation,less promiscuity,and more stable and reliable cell supply for nasal polyps research.