寻常性银屑病皮损中STAT3的表达及磷酸化STAT3的水平

Level of STAT3 and Phosphorylated STAT3 in the Lesions of Psoriasis Vulgaris

目的检测寻常性银屑病患者进行期斑块状皮损角质形成细胞(KCs)STAT3的表达和活化STAT3(p-STAT3)的水平,并探讨其意义。方法采用免疫组化Polymer酶标二抗法,检测21例银屑病皮损和20例取材于外科手术的正常皮肤KCs中STAT3的表达和p-STAT3的水平,并对银屑病患者进行PASI评分。结果银屑病皮损KCs中STAT3表达显著高于取材于外科手术的正常皮肤组织(后者呈低表达)(P<0.05),且其p-STAT3水平亦显著高于后者(后者呈低水平)(P<0.05);皮损KCs中STAT3表达和p-STAT3水平与银屑病PASI评分之间均存在正相关(r=0.633,0.680,P均<0.05),随着STAT3表达的增加、p-STAT3水平的升高,患者PASI评分也呈升高趋势。结论角质形成细胞STAT3的表达增加和活化在银屑病发病机制中可能起重要作用。

Objective To investigate the expression of STAT3 and the level of phosphorylated STAT3 （p-STAT3） in keratinocytes （KCs） of the plaque -like lesions of psoriasis vulgaris in progressive stage and discuss the rel- evant significance. Methods Immunohistochemical polymer enzyme-labeled secondary antibody method was used to detect the expression of STAT3 and the level of p-STAT3 in 21 cases of psoriatic lesions and 20 cases of normal skin tissues from surgery. The patients with psoriasis were assessed with the Psoriasis Area and Se- verity Index （PASI）. Results The expression of STAT3 and the level of p-STAT3 in KCs of psoriatic le- sions were both higher significantly than those in normal skin （ both P 〈 0.05 ）. 1＇he scores of STAT3 and p- STAT3 in KCs of psoriatic lesions both had positive correlations with PAS! （ r = 0.633, 0.680 respectively, both P 〈 0.05 ）. With the increasing expression of STAT3 and level of p-STAT3 in KCs, the scores of PASI ascended. Conclusions The increasing expression and the activation of STAT3 in KCs may be important in the pathogenesis of psoriasis.