五味子多糖对S180荷瘤小鼠红细胞免疫抗肿瘤的作用

Study of Fructus Schisandrae Chinensis Polysaccharides on Erythrocyte Immune Adherence Function and Anti-Tumor Effects in S180 Tumor-Bearing Mice

目的:探讨五味子多糖(FSCP)增强荷瘤小鼠红细胞结构稳定性与红细胞免疫黏附功能的抗肿瘤作用及其红细胞免疫调节机制。方法:实验动物随机分为正常对照组、生理盐水组、环磷酰胺(CP)组及FSCP高、中、低3个剂量组。用药7d后,眼球取血,获得红细胞悬液。采用激光共聚焦技术检测荷瘤小鼠红细胞Ca2+含量的变化;采用分光光度法检测红细胞膜唾液酸(SA)含量、红细胞膜封闭度;采用荧光偏振法检测红细胞膜脂流动性(LFU);采用花环法检测红细胞天然免疫黏附能力。结果:与生理盐水组比较,FSCP各组能降低荷瘤小鼠红细胞Ca2+的浓度(P<0.01),增加红细胞膜表面SA含量(P<0.01)以及红细胞膜自我封闭度(P<0.05或P<0.01)。高剂量FSCP可提高S180荷瘤小鼠红细胞膜流动性(P<0.05);增强S180荷瘤小鼠红细胞免疫黏附肿瘤细胞的能力(P<0.05),FSCP高剂量组免疫花环率达到24.17%。结论:FSCP可能通过改善S180荷瘤小鼠红细胞膜的功能状态,提高膜的稳定性,增强红细胞免疫黏附肿瘤细胞的能力,从而发挥其抗肿瘤作用。

Objective：To discuss the relationship between the structural stability of erythrocytes enhanced by Fructus Schisandrae Chinensis polysaccharides（FSCP） and erythrocyte immune adherence in tumor-bearing mice,and the immune regulatory mechanisms of the erythrocytes thereof.Methods：The KM mice were divided into control group,normal saline group,cyclophosphamide（CP） group and FSCP groups（high,middle and low doses）.After 7 d treatment,erythrocytes were obtained from eye-ball,and then red blood cell suspension was made.The change of [Ca2＋] was examined by laser scanning con-focal microscopy.The ectrophotometer method was used to measure the level of sialic acid（SA） and the degree of closure of erythrocytes membrane.The biological lipid membrane fluidity（LFU） was determined by the fluorescence polarization,and the natural ability of adhering tumor cells of erythrocytes was examined by rosette formation test.Results：In contrast to the normal saline group,the level of [Ca2＋] was decreased,and the level of SA in erythrocyte membrane,caused by FSCP in S180 tumor-bearing mice（P 0.01）.There was a higher LFU and degree of closure in erythrocyte membrane in model mice as well（P 0.05 or P 0.01）.FSCP enhanced the natural ability of red blood cells adhering tumor cells（P 0.05）at the ratio of 24.17 percent.Conclusion：FSCP showed an anti-tumor effect by improving the structure of erythrocyte membrane and enhancing the function of immune adherence of erythrocytes.