睾丸肉膜囊固定术在大鼠精索扭转模型中的应用

Application on the Dartos Pouch Orchiopexy in the Spermatic Cord Torsion Animal Models of Rats

目的:利用睾丸肉膜囊固定术建立大鼠精索扭转模型,为后续的进一步研究建立基础。方法:将42只SpragueDawley(SD)雄性大鼠,麻醉后分别取阴囊切口,扩张分离阴囊皮肤与肉膜层间隙,打开肉膜层,拉出睾丸、附睾,分离睾丸引带,将精索扭转后置于肉膜层外,维持精索位置不变,缝合肉膜层仅容精索通过,将睾丸、附睾置于肉膜囊内,缝合阴囊切口。结果:39只大鼠精索扭转动物模型建立成功。结论:笔者首创的利用睾丸肉膜囊固定术建立大鼠精索扭转模型,此建模方法安全可靠,没有损伤血睾屏障,为进一步的研究奠定了重要的基础。

Objective：To establish the experimental animal models of spermatic cord torsion in rats,using the dartos pouch orchiopexy.Methods：Dartos pouch orchiopexy was performed on forty-two rats to establish spermatic cord torsion experimental animal models of rats by transscrotal approach.Results：Thirty-nine rats survived the operation with successful acquisition of spermatic cord torsion while three rats died after anesthesia.Conclusions：The successful animal models of spermatic cord torsion are surgically established with dartos pouch orchiopexy.