Livin和Survivin基因在成人急性淋巴细胞白血病中的表达及临床意义

Gene Expression of Livin and Survivin in Adult Patients with Acute Lymphoblastic Leukemia and Its Clinical Significance

本研究旨在探讨凋亡抑制蛋白家族成员livin和survivin基因在成人急性淋巴细胞白血病(ALL)中的基因表达及其临床意义。应用半定量逆转录-聚合酶链反应(RT-PCR)方法检测95例成人ALL患者(包括52例初治ALL患者,23例治疗后复发的ALL患者以及20例完全缓解的ALL患者)骨髓中和20例健康者外周血中的livin和survivinmRNA表达情况。结果表明,成人ALL患者初治组、复发组中livin和survivin的表达均明显高于缓解组和正常对照组;初治成人ALL患者中,livin和survivin的表达与否与患者的年龄、性别、分型、初诊时白细胞水平均无明显相关;初治成人ALL患者中,livin、survivin表达阳性的患者缓解率明显低于表达阴性的患者;livin和survivin在成人ALL初治组中的表达无明显相关性。结论:livin和survivin可能参与成人ALL的发生与发展;livin和survivin高表达可能是成人ALL预后不良指标;livin和survivin在成人ALL中可能没有协同表达作用。

This study was aimed to investigate the expressions of livin and survivin in adult patients with acute lymphoblastic leukemia （ALL） and clinical significance. The expressions of livin and survivin mRNA in bone marrow mononuclcar cells of 95 adult ALL patients including 52 de novo patients, 23 relapsed patients and 20 patients with complete remission （CR）, and 20 healthy adults as normal controls were detected by using RT-PCR. The results indicaded that the expression of livin and survivin mRNA in de novo and relapsed patients was higher than that in normal controls and patients with CR. There were no significant relation of expression level with clinical features such as age, sex, type and de novo leukocyte level. The CR rate in de novo adult ALL patients with positive expression of livin and survivin was lower than that in adult ALL patients with negative gene expression. No relation of mRNA expression between livin and survivin was found in de novo adult ALL patients. It is concluded that genes livin and survivin may be involved in the pathogenesis and progression of adult ALL. Overexpression of livin or survivin may show poor progression. There is no relation of expression between genes livin and survivin in de novo adult ALL patients.