急性脊髓损伤模型大鼠白细胞介素1β和核因子κB的表达

Expression of interleukin-1 β and nuclear factor kappa B in rat models of acute spinal cord injury

背景:在脊髓损伤后的继发性损伤过程中,白细胞介素1β参与刺激其他细胞因子和损伤介质的合成。目的:观察白细胞介素1受体拮抗剂对急性脊髓损伤模型大鼠损伤脊髓白细胞介素1β与核因子κB表达的影响。方法:采用改良Allen法建立SD大鼠急性脊髓损伤模型,造模后分别在损伤处敷含白细胞介素1受体拮抗剂或仅有生理盐水的明胶海绵,于脊髓损伤1,48,72h取损伤段脊髓标本,免疫组织化学染色检测白细胞介素1β与核因子κB的表达。结果与结论:经白细胞介素1受体拮抗剂治疗后,损伤脊髓组织白细胞介素1β和核因子κB的表达均显著降低。说明白细胞介素1受体拮抗剂可通过抑制白细胞介素1β和核因子κB的表达,减轻局部炎症反应,对急性脊髓损伤大鼠损伤段脊髓发挥保护作用。

BACKGROUND：During the process of secondary spinal cord injury, interleukin-1β participates in stimulation of other cytokines and synthesis of injury medium.OBJECTIVE：To investigate the effects of interleukin-1 receptor antagonist on the expression of interleukin-1β and nuclear factor-κB in rat models of acute spinal cord injury. METHODS：Sprague-Dawley rat models of acute spinal cord injury were established by modified Allen method. After modeling, the injured spinal cord tissue was spread gelatin sponge containing interleukin-1 receptor antagonist or normal saline. At 1, 48, and 72 hours postoperatively, the injured spinal cord tissue samples were harvested for detection of interleukin-1β and nuclear factor κB by immunohistochemical method. RESULTS AND CONCLSUION：After interleukin-1 receptor antagonist treatment, interleukin-1β and nuclear factor κB expression was greatly decreased. These findings suggest that interleukin-1 receptor antagonist can alleviate local inflammatory reaction by inhibiting interleukin-1β and nuclear factor κB expression, exerting protective effects on injured spinal cord segment in rat models of acute spinal cord injury.