期刊文献+

带气囊导管构建脊髓缺血模拟再灌注与缺血分离模型 被引量:1

Establishment of rat models of reperfusion and ischemia separation using a balloon catheter
下载PDF
导出
摘要 背景:采用带有气囊的导管急性压迫脊髓缺血模拟人类损伤可以造成再灌注与缺血分离的动物模型。目的:应用免疫组化和生物化学分析方法观察不同缺血时间窗处理对损伤脊髓的影响。方法:SD大鼠36只,随机分为假手术组,带气囊导管造成大鼠脊髓缺血10,30,45,60,90min组。结果与结论:再灌注48h后,随着缺血时间的延长,脊髓前角神经元坏死和凋亡逐渐加重,丙二醛水平逐渐增加,超氧化物歧化酶活性逐渐下降,大鼠的神经行为学病症加重。提示用带气囊的导管建立缺血再灌注大鼠模型成功,再灌注后大鼠脊髓的损伤随缺血时间的延长而加重。 BACKGROUND:Animal models of reperfusion and ischemia separation can be established by insertion of a balloon catheter into the spinal cord to minic human acute spinal injury. OBJECTIVE:To investigate the effects of different ischemic time windows on spinal injury using immunohistochemical and biochemical methods. METHODS:Thirty-six Sprague-Dawley rats were randomly divided into six groups:sham surgery, ischemia 10, 30, 45, 60 and 90 minutes. All rats were subjected to ischemia at the corresponding ischemia time and reperfused for 48 hours.Then, rat spinal cords were examined by immunohistochemical and biochemical analyses.RESULTS AND CONCLUSION:After 48 hours of reperfusion, with the elongation of ischemia time, spinal cord anterior horn neuron necrosis and apoptosis was gradually aggravated, malondialdehyde level was gradually increased, superoxide dismutase activity was gradually decreased, and rat neuroethological symptoms were aggravated. These findings suggest that the rat models of ischemia-reperfusion injury were successfully established using a balloon catheter,and the injury of the spinal cords was deteriorated increasingly with the elongation of ischemia time.
出处 《中国组织工程研究与临床康复》 CAS CSCD 北大核心 2011年第28期5189-5192,共4页 Journal of Clinical Rehabilitative Tissue Engineering Research
  • 相关文献

参考文献8

二级参考文献29

  • 1施瑞华,陈图兴.自由基清除剂SOD和CAT在肝纤维化中的预防和保护作用[J].中华消化杂志,1993,13(2):84-86. 被引量:8
  • 2姚煜.乙型病毒性肝炎血清丙二醛临床观察[J].临床肝胆病杂志,1995,11(2):104-105. 被引量:2
  • 3靳安民,徐印坎.高压氧对急性脊髓损伤诱发电位影响的实验观察[J].中华骨科杂志,1989,9(2):131-134. 被引量:8
  • 4Marcia B. New view of spinal cord injury[J]. Science, 1996, 274 : 1466.
  • 5Sakurai M, Hayashi T, Abe K, el al. Delayed and seleclive motot neuron death after transient spinal cord ischemia: a role of apoptosis?[J].Journal of Thoracic & Cardiovascular Surgery, 1998. 115(6):1310-1315.
  • 6Kiyoshima T, Fukuda S, Malsumoto M, et al. Lack of evidence for apoptosis as a cause of delayed onset paraplegia after spinal cord ischemia in rahbits[J].International Anesthesia Research Society, 2003, 96(3) :839- 846.
  • 7赵立,贾连顺,李涛,孙锡超.L-精氨酸治疗脊髓损伤的实验研究[J].中华创伤杂志,1997,13(3):164-166. 被引量:6
  • 8Panahian N, Maines MD.Site of injury-directed induction of heme oxygenase-1 and-2 in experimental spinal cord injury and differential functions in neuronal defense mechanisms?[J] J Neurochem, 2001,76(2):539-554.
  • 9Crowe MJ, Shuman SL, Masters JN et al. Morphological evidence suggesting apoptosis nuclei in spinal cord injury[J]. Soc Neursci Abstr, 1995,21:232.
  • 10Wang GY, Hirai K, Shimada H et al. Behavior of axons swchwann cells and perineurial cells in nerve regeneration with in implanted nerve grafts[J]. Brain Res,1992,583(1-2):216-226.

共引文献56

同被引文献28

  • 1管玉龙,刘锋,董培青,万彩红,杨璟.脊髓缺血损伤动物模型的建立[J].中国体外循环杂志,2006,4(1):46-48. 被引量:7
  • 2Iwamoto S,Higashi A,Ueno T,et al.Protective effect of sivelestat Sodium hydrate (ONO-5046) on ischemic spinal cord injury[J].Interact Cardiovasc Thorac Surg,2009,8(6): 606-609.
  • 3Feezor RJ,Martin TD,Hess PJ,et al.Extent of aortic coverage and incidence of spinal cord ischemia after thoracic endovascular aneurysm repair[J].Ann Thorac Surg,2008,86(6): 1809-1814.
  • 4Zivin JA,DeGirolami U.Spinal cord infarction: a highly reproducible stroke model[J].Stroke,1980,11(2): 200-202.
  • 5Herlambang B,Orihashi K,Mizukami T,et al.New method for absolute spinal cord ischemia protection in rabbits[J].J VASC SURG,2011,54(4): 1109-1116.
  • 6Kim J,Hwang J,Huh J,et al.Acute normovolemic hemodilution can aggravate neurological injury after spinal cord ischemia in rats[J].Anesth Analg,2012,114(6): 1285-1291.
  • 7Wang Z,Yang W,Britz GW,et al.Development of a simplified spinal cord ischemia model in mice[J].J Neurosci Methods,2010,189(2): 246-251.
  • 8Kakimoto M,Kawaguchi M,Sakamoto T,et al.Evaluation of rapid ischemic preconditioning in a rabbit model of spinal cord ischemia[J].Anesthesiology,2003,99(5): 1112-1117.
  • 9Hori M,Aoki S,Oishi H,et al.Utility of time-resolved three-dimensional magnetic resonance digital subtraction angiography without contrast material for assessment of intracranial dural arterio-venous fistula[J].Acta Radiol,2011,52(7): 808-812.
  • 10Li MH,Li YD,Tan HQ,et al.Contrast-free MRA at 3.0 T for the detection of intracranial aneurysms[J].Neurology,2011,77(7): 667-676.

引证文献1

二级引证文献12

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部