带气囊导管构建脊髓缺血模拟再灌注与缺血分离模型

Establishment of rat models of reperfusion and ischemia separation using a balloon catheter

背景:采用带有气囊的导管急性压迫脊髓缺血模拟人类损伤可以造成再灌注与缺血分离的动物模型。目的:应用免疫组化和生物化学分析方法观察不同缺血时间窗处理对损伤脊髓的影响。方法:SD大鼠36只,随机分为假手术组,带气囊导管造成大鼠脊髓缺血10,30,45,60,90min组。结果与结论:再灌注48h后,随着缺血时间的延长,脊髓前角神经元坏死和凋亡逐渐加重,丙二醛水平逐渐增加,超氧化物歧化酶活性逐渐下降,大鼠的神经行为学病症加重。提示用带气囊的导管建立缺血再灌注大鼠模型成功,再灌注后大鼠脊髓的损伤随缺血时间的延长而加重。

BACKGROUND：Animal models of reperfusion and ischemia separation can be established by insertion of a balloon catheter into the spinal cord to minic human acute spinal injury. OBJECTIVE：To investigate the effects of different ischemic time windows on spinal injury using immunohistochemical and biochemical methods. METHODS：Thirty-six Sprague-Dawley rats were randomly divided into six groups：sham surgery, ischemia 10, 30, 45, 60 and 90 minutes. All rats were subjected to ischemia at the corresponding ischemia time and reperfused for 48 hours.Then, rat spinal cords were examined by immunohistochemical and biochemical analyses.RESULTS AND CONCLUSION：After 48 hours of reperfusion, with the elongation of ischemia time, spinal cord anterior horn neuron necrosis and apoptosis was gradually aggravated, malondialdehyde level was gradually increased, superoxide dismutase activity was gradually decreased, and rat neuroethological symptoms were aggravated. These findings suggest that the rat models of ischemia-reperfusion injury were successfully established using a balloon catheter,and the injury of the spinal cords was deteriorated increasingly with the elongation of ischemia time.