改构型酸性成纤维细胞生长因子干预慢性衰老模型大鼠脑及肝组织和血液ATP酶的活力

Effects of modified acidic fibroblast growth factor on the ATPase activities in blood, brain and liver tissue of chronic aging model rats

背景:有研究表明改构型酸性成纤维细胞生长因子是多功能生长因子,但其抗衰老作用至今尚未有报道。目的:观察改构型酸性成纤维细胞生长因子对D-半乳糖致衰老大鼠脑组织、肝组织及红细胞膜Na+-K+-ATP酶活力和Ca2+-Mg2+-ATP酶活力的影响。方法:将Wistar大鼠采用皮下注射D-半乳糖建立衰老模型,建模成功后随机分为模型组、生理盐水对照组和改构型酸性成纤维细胞生长因子组,另设正常对照组。改构型酸性成纤维细胞生长因子组按12μg/kg剂量肌肉注射改构型酸性成纤维细胞生长因子,生理盐水对照组肌肉注射等量的生理盐水,模型组不干预。结果与结论:与正常对照组相比,模型组大鼠脑组织、肝组织及红细胞膜Na+-K+-ATP酶活力和Ca2+-Mg2+-ATP酶活力均明显著降低(P<0.01)。改构型酸性成纤维细胞生长因子组脑组织、肝组织及红细胞膜Na+-K+-ATP酶活力和Ca2+-Mg2+-ATP酶活力均明显高于模型组和生理盐水对照组(P<0.05)。结果提示,改构型酸性成纤维细胞生长因子能提高衰老大鼠脑组织、肝组织及红细胞膜Na+-K+-ATP酶活力和Ca2+-Mg2+-ATP酶活力,具有延缓衰老作用。

BACKGROUND：Modified acidic fibroblast growth factor （maFGF） is a multifunctional grows factor, but its anti-aging actions remains unclear. OBJECTIVE：To study the effects of maFGF on the activities of Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase in brain tissue, liver tissue and red blood cells of D-galactose-induced aging model rats. METHODS：The aging model in Wistar rats was established by subcutaneous injection of D-galactose. The successful models were randomly divided into model group, normal saline group and maFGF treatment group. Rats in the latter two groups received a 12-μg/kg intramuscular injection of normal saline and maFGF, respectively. Model group received no treatment. In addition, normal control group was set. RESULTS AND CONCLUSION：The activities of Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase in brain tissue, liver tissue and red blood cells were significantly decreased compared with normal control group （P 0.01）. After maFGF intervention, the activities of Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase were significantly higher than those in the normal saline and model groups （P 0.05）. The maFGF can increase the activities of Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase in brain tissue, liver tissue and red blood cells and delay the ageing in D-galactose-induced aging model rats.