野百合碱诱导大鼠肺动脉高压模型的建立

Establishment of rat models of pulmonary hypertension induced by monocrotaline

背景:目前尚缺乏简单易行、实用、操作性强的肺动脉高压动物模型。目的:建立一种实用的注射野百合碱诱导的肺动脉高压动物模型。方法:采用一次性皮下注射野百合碱60mg/kg的方法制备SD大鼠肺动脉高压模型。结果与结论:野百合碱注射后第1,2,3,4周,大鼠平均肺动脉压明显升高,右心室肥厚明显。光镜下可见肺小血管肌化程度增强,相对中膜厚度增加,肺血管密度减少,以上症状均随野百合碱注射时间的延长逐渐加重。证实此方法建立的大鼠肺动脉高压模型造模成功。

BACKGROUND：The fibrin glue or OB glue can be used in the repair of peripheral nerve damage, but their colloidal structure and underlying mechanism are entirely different. OBJECTIVE： To contrastively analyze the effect of fibrin glue or OB glue combined with chitosan-collagen conduit for repairing rabbit facial nerve damage. METHODS：Chinese rabbits were used to establish the injury model in right facial nerve of rabbits, which were divided into three groups randomly：microsurgery anastomosis group：the nerve stump contraposition and adventitia in situ anastomosis were performed;fibrin glue group and OB glue group were respectively treated with fibrin glue and OB glue based on microsurgery anastomosis. General observation, electrophysiological study, histological study and image analysis were performed at 16 weeks postoperatively. All results were used to evaluate the nerve regeneration.RESULTS AND CONCLUSION：The chitosan-collagen conduit was obviously degraded at 16 weeks postoperatively and they also restrained the formation of fibrous connective tissue around anastomotic stoma. The functional recovery of nerve muscles was good in three groups, the motor nerve action potential and the compound muscle action potential of orbicularis oris were analyzed, which showed no significant difference between them （P 0.05）. The recovery rate of regenerated axons of three groups had no significant difference （P 0.05）, but the axonal regeneration rate in the fibrin glue group and OB glue group was higher than in microsurgery anastomosis group （P 0.05 or 0.01）, reached a peak at OB glue group. Chitosan-collagen conduit have excellent biocompatibility, and combined with fibrin glue or OB glue certainly result in repairing injured nerves, but the fibrin glue is more suitable for operative nerve injury.