骨骼肌细胞αB-晶状体蛋白基因在大强度离心运动后的表达

Expression of alpha B-crystallin mRNA of skeletal muscle cells after high-intensity eccentric exercise

背景:骨骼肌含有被称为"分子伴侣"的小热休克蛋白αB-晶状体蛋白,有可能在肌肉运动中具有重要的生理功能。目的:观察离心运动后骨骼肌细胞αB-晶状体蛋白基因表达。方法:将成年雄性Wistar大鼠随机分为安静对照组和运动后即刻组,运动后24h组。安静对照组正常喂养,两个运动组进行一次性大负荷离心运动,分别取对照组腓肠肌和运动组运动后0和24h的腓肠肌,使用冰冻切片原位杂交法检测αB-晶状体蛋白基因表达情况。结果与结论:骨骼肌细胞αB-晶状体蛋白基因在对照组呈低水平表达,在运动后即刻和运动后24h组均呈较高表达(P<0.05),αB-晶状体蛋白基因表达杂交信号主要位于肌细胞膜下。结果证实,大强度离心运动可诱导骨骼肌细胞αB-晶状体蛋白基因表达增高。

BACKGROUND：Skeletal muscle is called ＂molecular chaperones＂ the small heat shock protein αB-crystallin protein in the exercise training may have important physiological functions. OBJECTIVE：To examine the mRNA expression of αB-crystallin in skeletal muscle after eccentric exercise. METHODS：Adult male Wistar rats were randomly divided into control group, immediately after exercise group and 24 hours after exercise group. Control group received normal feeding. Exercise groups experienced once high-intensity eccentric exercise. After exercise, gastrocnemius muscle from excise groups at 0 and 24 hours after excise and those from control group were removed. The gastrocnemius muscles were processed for cryosection in situ hybridization to measure αB-crystallin mRNA expression. RESULTS AND CONCLUSION：Compared with control group which had a weak level of labeling, the hybridization signal of αB-crystallin mRNA was obviously enhanced near muscle cell membrane in immediately after exercise group and 24 hours after exercise group （P 0.05）. Results suggest that αB-crystallin mRNA expression was up-regulated after high-intensity eccentric exercise.