母子分离应激对complexin Ⅱ基因缺陷鼠快速移动行为的影响

Effects of neonatal isolation stress on hyperlocomotion of complexin Ⅱ knockout mouse

本文旨在探讨母子分离应激对complexin Ⅱ基因缺陷(Cplx2-/-)小鼠快速移动行为的影响。实验分4组:应激Cplx2-/-组、应激Cplx2+/+组、无应激Cplx2-/-组和无应激Cplx2+/+组。无应激组小鼠正常饲养,应激组小鼠从出生后第2日至第21日每天施加母子分离应激。小鼠生长到第4周时,利用聚合酶链反应技术检测其基因型。4组小鼠腹腔注射激动剂去氧麻黄碱或生理盐水后,采用EthoVision系统对小鼠快速移动行为进行记录。结果显示,应激Cplx2-/-组、应激Cplx2+/+组、无应激Cplx2-/-组和无应激Cplx2+/+组注射去氧麻黄碱后,与生理盐水对照组相比,均出现快速移动行为不同程度的增多(P<0.01);与无应激Cplx2-/-组相比较,应激Cplx2-/-组注射去氧麻黄碱后快速移动行为增多更为明显(P<0.001);与应激Cplx2+/+组相比,应激Cplx2-/-组注射去氧麻黄碱后的快速移动行为增多更为明显(P<0.001)。本实验证实小鼠的行为可能受母子分离应激和Cplx2基因等因素的多重影响。

The aim of this study was to investigate the influence of neonatal isolation stress on hyperlocomotion in complexin Ⅱ knockout mouse （Cplx2-/- ）. The mice were randomly divided into 4 groups： Cplx2-/- with stress, Cplx2 ＋/＋ with stress, Cplx2-/- without stress and Cplx2 ＋/＋ without stress. Isolation stress was employed on the pups of stress groups from the 2nd day after the postnatal to the 21st day. The PCR was used to determine the gene type and the hyperlocomotion test was employed to detect the change of animal behavior after methamphetamine or saline injection （i.p.）. The results showed that the animals of all groups increased their movement after injection of 0.2 mg/kg methamphetamine in different levels （P 0.01）, compared with those injected with saline. The Cplx2-/- mouse with stress revealed a significant increase in the distance of free movement after injection of 0.2 mg/kg methamphetamine compared with the knockout mouse without stress （P 0.001）. When Cplx2-/- mouse with stress was compared with wild type with stress, Cplx2-/- mouse with stress had more movement （P 0.001）, indicating that Cplx2 has effect on the hyperlocomotion as well. These results suggest an involvement of stress and Cplx2 in the movement behavior of mice.