骨髓间充质干细胞移植减轻衰老大鼠肠损伤

Bone marrow mesenchymal stem cells transplantation for the reduction of intestine injury in aging rat

目的探讨骨髓间充质干细胞(MSCs)在D-半乳糖制备的衰老大鼠小肠损伤中的作用。方法 SD大鼠30只,随机均分为3组:对照组、衰老模型组和MSCs防治组。给衰老模型组大鼠每日皮下注射D-半乳糖400 mg/kg,连续4个月。MSCs防治组在衰老模型制备成功后,给予尾静脉输注3×106个MSCs。分别采用硫代巴比妥酸和黄嘌呤氧化法检测小肠组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性;观察3组大鼠小肠组织结构的差异,并用表达绿色荧光蛋白(GFP)的腺病毒载体标记MSCs,以确定MSCs的植入情况。结果 GFP标记的MSCs移植给大鼠后,能向小肠组织迁移并存活。MSCs防治组SOD活性为133.7±3.6 U/mL,显著高于衰老模型组的105.1±4.3 U/mL(P<0.01);而MDA含量为5.9±0.1 nmol/mL,显著低于衰老模型组的6.9±0.1 nmol/mL(P<0.01)。模型组大鼠肠道黏膜损伤严重,而MSCs防治组大鼠的小肠损伤有明显修复。结论骨髓间充质干细胞能够一定程度上减轻D-半乳糖致衰老大鼠的小肠损伤。

Objective To observe the effect of bone-marrow mesenchymal stem cells on intestine in the aging rats induced by D-galactose.Methods The aging model group were injected with D-galactose for 4 months to establish the aging rats.The contents of superoxide dismutase（SOD） and malondialdehyde（MDA） in intestine was measured wtih hydroxylamine and chromatometry respectively.The pathological morphological changes of intestine were observed.MSCs were transfected by adenovirus vectors expressing green fluorescent protein（GFP） to construct GFP-MSCs,and the migration of MSCs in intestine was examined.Results MSCs marked by GFP migrated into the intestine.Compared with the aging model group,the activity of SOD was significantly lower,the content of MDA increased significantly in MSCs group,and there were significant differences：（133.7±3.6）,（105.1±4.3）U/mL,P〈0.01;（5.9±0.1）,（6.9±0.1）（nmol/mL）,P〈0.01.The intestinal mucosa injury is serious in aging model group rats;and the intestine pathological injury had obvious repaired in MSCs group,compared with aging model group.Conclusion Transplantation of mesenchymal stem showed a protective effect on intestine damage induced by D-galactose.