乳腺癌细胞株两种原代培养方法的比较

A comparison of two methods in primary cultures of breast cancer cells in vitro

目的探讨乳腺癌细胞株原代培养的方法。方法取"人乳腺-人乳腺"小鼠模型的乳腺癌原位灶,用组织块培养法和细胞培养法分别对其进行原代培养,并对两种培养方法的结果进行比较。结果两种方法均能得到乳腺癌原代细胞,组织块培养法的成功率为90.0%,细胞培养法的成功率为20.0%。结论组织块培养法是较好的原代培养方法。

Objective To study the method of primary culture of human breast cancer cells.Methods The tissue was separated from the orthotopic tumor formation in the human breast tissue-derived orthotopic and metastatic（BOM） mouse model of breast cancer.Tissue explants were cultured by two methods,tissue culture method and cell culture method.The results reporded by the two techniques were analyzed and compared.Results The breast carcinoma cells could be cultured by these two methods with a success rate of 90% in the tissue culture method and that of 20% in the cell culture method.Conclusion Tissue culture method is better than cell culture method in getting primary culture of human breast cancer cells.