姜半夏乙醇提取物对人胃癌SGC7901细胞增殖和凋亡的影响

Effects of Enthanol Extract from Rhizome Pinelliae Preparata on Proliferation and Apoptosis of Human Gastric Cancer Cells SGC7901

目的:观察姜半夏乙醇提取物对人胃癌SGC7901细胞增殖和凋亡的影响。方法:不同浓度姜半夏乙醇提取物(终浓度为1mg/ml,0.5mg/ml,0.25mg/ml,0.125mg/ml)处理SGC7901细胞后,倒置相差显微镜下观察细胞的形态学变化;通过甲基噻唑基四唑法检测细胞增殖状况、描绘生长曲线,使用紫外分光光度法观察药物干预后细胞ATP酶活力;AnnexinV-异硫氰酸荧光素(fluorescein isothiocyanate,FITC)/碘化丙啶(propidium iodide,PI)双标记法流式细胞术检测姜半夏乙醇提取物对SGC7901细胞诱导凋亡的情况。结果:不同浓度姜半夏乙醇提取物均能不同程度地抑制人胃癌SGC7901细胞的增殖;在姜半夏乙醇提取物诱导细胞后细胞发生了边缘毛刺、体积缩小等形态学变化,同时可见细胞折光度和贴壁能力下降;AnnexinV-FITC/PI双标记法检测显示姜半夏乙醇提取物可诱导细胞发生凋亡;细胞总ATP酶活力在药物干预72小时后出现明显下降;并且随着药物浓度增加细胞凋亡率、细胞形态异常改变以及ATP酶活力抑制作用均呈上升趋势。结论:姜半夏乙醇提取物可抑制人胃癌SGC7901细胞的增殖,促进其凋亡,抑制细胞ATP酶活力。

Objective： To observe the effects ofenthanol extract from Rhizome Pinelliae Preparata with different concentrations on proliferation and apoptosis of human gastric cancer cells SGC7901. Methods： After coculturing SGC7901 cells with enthanol extract from Rhizome Pinelliae Preparata （1 mg/ml, 0.5mg/ml, 0.25mg/ml, 0.125mg/ml）, inverted microscope was utilized to observe morphological changes; The cell viability was evaluated with MTT chromatometry and cell growth curve was generated; The activity of total ATPase of each groups were evaluated with UV spectrophotometric determination; Annexin V-fluorescein isothiocyanate/propidium io- dide double label method was used to detect apoptosis rate of SGC7901 cells. Results： The proliferation of SGC7901 cells can be sharply inhibited by enthanol extract from Rhizome Pinelliae Preparata with different concentration; After treating with enthanol extract from Rhizome Pinelliae Preparata, cellular morphology changed, including brim of cell wall roughening and cell volumes shrinkening, which result in a decline in diopter and the abilty of adhenrence to plate surface; It was observed by flow cytometry that enthanol extract from Rhizome PineUiae Preparata can induced SGC7901 cells apoptosis. The activity of total ATPase decreased significantly after coculturing SG7901 cells with drugs for 72h. The all effect of enthanol extract from Rhizome Pinelliae Preparata can be enhanced with the increase of its dose. Conclusion： Enthanol extract from Rhizome Pinelliae Preparata can inhibit the SGC7901 cells proliferation and induce cell apoptosis, decrease the activity of total ATPase.