原儿茶酸对帕金森模型鼠脑组织抗氧化能力的影响

Effects of Protocatechuic acid on antioxidative abilities in the brains of rotenone-induced Parkinson's Disease mice

目的:观察原儿茶酸对鱼藤酮诱导的帕金森模型小鼠脑组织中相关抗氧化酶活性的影响,为原儿茶酸治疗帕金森病提供理论依据。方法:选用昆明小鼠为实验动物,连续5周腹腔注射鱼藤酮(1mg/kg.d)建立帕金森模型,之后2周分别注射原儿茶酸(5mg/kg.d)和阳性药物美多芭(125mg/kg.d)。应用生物化学方法,观察原儿茶酸对帕金森模型小鼠中脑和纹状体内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)活力以及脂质过氧化物丙二醛(MDA)含量的影响。结果:实验数据显示原儿茶酸可提高帕金森模型小鼠中脑和纹状体SOD、CAT、GSH-PX活力,降低MDA含量。同时病理切片染色显示原儿茶酸可减轻鱼藤酮诱导的脑组织损伤。结论:原儿茶酸具有神经保护作用,保护机制可能是提高脑组织内源性抗氧化酶活力,减少体内自由基的产生,进而减轻了脑组织的病理损伤。

Objective： The aim of this paper was to investigate the effects ofprotocatechuic acid （PCA） on the antioxidative abili- ties in the brains of rotenone-induced Parkinson＇s disease （PD） mice. Methods： KunMing mice were used as experimental animals and intrapefitoneally injected with rotenone （lmg/kg.d） for 5 weeks to establish PD model. From the fifth week, the PCA and positive group mice were subcutaneously injected with PCA （5 mg/kg.d） and Madopar （125mg/kg.d） for 2 weeks. KunMing mice were used as experimental animals and intraperitoneal injected with rotenone （lmg/kg.d） for 5 weeks to establish PD model. From the fifth week, the PCA and positive group mice were subcutaneously injected with PCA （5 mg/kg.d） and Madopar （125mg/kg.d） for 2 weeks. The activities of superoxide dismutase （SOD）, catalase （CAT） and glutathione peroxidase （GSH-PX） and the content of malondialdehyde （MDA） in the midbrain and striatum of mice were assayed using biochemical methods. Results： The results showed that PCA significantly increased SOD, CAT, GSH-PX activities and reduced MDA content in the brains of rotenone induced PD mice. Furthermore, pathological staining showed that PCA reduced rotenone-induced brain damage. Conclusions： These results indicated that PCA had a neuroprotective function, and its protective effects on mice brain were achieved at least partly by promoting endogenous antioxidant enzymatic activities, reducing the production of free radicals and brain damage.