丁咯地尔和α-硫辛酸对糖尿病大鼠肾功能的保护作用研究

Protective effect of Buflomedil and Alpha lipoic aci on kidney function of diabetic rats

目的:研究丁咯地尔和α-硫辛酸(ALA)对糖尿病(DM)大鼠肾功能的保护作用。方法:雄性SD大鼠用链脲佐菌素(60 mg/kg)腹腔注射诱导DM。大鼠随机分为健康组(NC)、DM组(DMC)、DM+ALA组(ALA组)、DM+丁咯地尔组(丁咯地尔组)。ALA组和丁咯地尔组每天给予ALA 100 mg/kg灌胃和丁咯地尔(0.1 g/kg)腹腔注射,NC组和DMC组大鼠给予等量生理盐水每日灌胃。干预4周后,测观察治疗后大鼠血糖、尿素氮、血肌苷,内生肌苷清除率、24小时尿蛋白排泄率,比色法检测血清抗氧化酶和丙二醛(MDA)含量。结果:①丁咯地尔和α-硫辛酸降低糖尿病大鼠的尿素氮、血肌苷、24h尿白蛋白排泄率、丙二醛,增加超氧化物歧化酶②两种药物联合用药具有协同作用。结论:丁咯地尔和ALA可能通过抑制机体氧化应激水平,减低早期DM大鼠肾脏损害。

Objective： To investigate the protective effect ofBuflomedil and α -lipoid acid on renocortieal of diabetic rats. Methods： Experimental diabetes model was induced by intraperitoneal injection of streptozotocin （60 mg/kg）in male Sprague Dawley rats. The rats were randomly divided into following 5 groups： normal control（NC）, diabetes mellitus control（DMC）, diabetics treated with Buflomedil （0.1 g/kg） intraperitoneal injection, and diabetics treated with a lipoid acid （ALA, 100 mg/kg/day）. After 4 weeks treatment, blood glucose, 24 h urinary protein （24 h UP）, serum creatinine, and blood urea nitrogen were detected as renal function indexes. And the malondi- aldehyde （MDA） and antioxidant enzymes was evaluated with colorimetry method. Results： ①Buflomedil and α -lipoid acid decrease blood urea nitrogen, serum ereatinine, and 24 h urinary protein （24 h UP）, serum creatinine, MDA and blood urea nitrogen; increase Serum superoxide dismutase②two drugs have a synergistic effect in combination. Conclusion： Buflomedil and ALAmay could inhibiting the oxidative stress in renal cortex of diabetic rats, so that reducing the early kidney injury.