口服卡介菌治疗实验性自身免疫性脑脊髓炎的研究

Studies on the therapeutic effect of oral BCG on experimental autoimmune encephalomyelitis

目的:观察口服卡介菌对实验性变态反应性脑脊髓炎(EAE)的治疗效果。方法:制备EAE大鼠模型,随机分为BCG高、中、低剂量组和PBS对照组,每组各15只,对大鼠治疗后的临床症状及病理组织学进行评估,提取脾脏淋巴细胞,流式细胞术检测T淋巴细胞亚群,3H-TdR掺入法检测淋巴细胞增殖能力。结果:BCG组EAE大鼠与对照组相比,临床症状减轻,发病时间延迟,炎性细胞浸润数减少;急性期,口服BCG各组CD4+、CD8+T细胞的数量随剂量增加而增加,缓解期CD4+、CD8+T细胞数量减少;口服BCG可促进EAE大鼠T淋巴细胞增殖能力;高、中剂量组上述变化均较其它分组明显。结论:口服BCG可很好的诱导免疫耐受,延迟EAE发病,减轻炎症反应,改善临床症状。

Objective： To investigate the therapeutic effect of oral BCG on experimental autoimmune encephalomyelitis. Methods： The EAE rats were randomly divided into four groups： high dose BCG group, medium dose BCG group, low dose BCG group and PBS control group. Clinical score and HE staining were respectively used for clinical symptom assessment and pathological examination. Lymphocytes were extracted from spleen, then T-type lymphocyte subsets were tested by flow cytometry and lymphocyte proliferation was tested by assay of 3H-TdR incorporation. Results： Compared with control group, clinical symptom was relieved, the disease onset time was delayed, and the number of inflammatory cell infiltration was reduced in oral BCG group. The number of CD4^＋ and CD8^＋ T lymphocytes of oral BCG group was increased in a dose-dependent manner during acute phase, but decreased during remission phase. Oral BCG inhibited the proliferation of T lymphocytes. These changes in high and medium dose group were more obvious than those in low dose and control groups. Conclusion： Oral BCG could induce immune tolerance, delay the onset time, relieve inflammatory response and improve the clinical symptoms of EAE.