摘要
目的:优化营养保健甘薯汁的制备工艺。方法:本研究以甘薯为原料,在加酶量、作用时间、反应温度、pH及底物浓度五个单因素试验的基础上采用响应面分析法,以甘薯浆中还原糖量为评价指标,对耐高温α-淀粉酶酶解甘薯浆中淀粉的最佳工艺进行了研究,并利用统计学方法建立了耐高温α-淀粉酶酶解甘薯浆中淀粉的二次多项数学模型。结果:最佳酶解条件为:加酶量480U/g,作用时间90min,反应温度77℃,pH值6.0,底物浓度2.6g/10ml。结论:在最佳酶解条件下,甘薯中还原糖最大估计值为13.97345%,实测值为(13.968±0.05)%。
Objective： To optimize the preparation technology of Nutritional sweet potato juice sweet potato. Method： Based on single-factor experiments including factors such as thermostable α -amylase dosage, time, temperature, pH and substance concentration in sweet potato starch hydrolysis, the response surface methodology was adopted with reducing sugar amount as evaluating indicator to optimize the process. Results： The optimum hydrolysis parameters are enzyme dosage 480 U/g, time 90 min, temperature 77℃, pH 6.0 and substance concentration 2.6g/10ml. Conclusions： The value of reducing sugar is 13.97345 % at the optimum conditions, and the measured value is （13.968± 0.05） %.
出处
《现代生物医学进展》
CAS
2011年第17期3270-3274,3292,共6页
Progress in Modern Biomedicine
关键词
甘薯
淀粉
耐高温Α-淀粉酶
响应面
Sweet potato
Starch
thermostable α -amylase
Response surface methodology
