mTOR、eIF4E、4E-BP1在宫颈癌组织中的表达及临床意义

Study on expression of mTOR、eIF4E and 4E - BP1 and their clinical significance in human cervical carcinoma

目的 探讨mTOR、eIF4E、4E-BP1在宫颈癌发生发展中的作用和相关性,为预后判断提供理论依据.方法 采用免疫组织化学SP法检测mTOR、eIF4E、4E-BP1在53例宫颈癌组织、15例宫颈上皮内瘤变组织和13例正常宫颈组织中的表达.结果 （1）mTOR、eIF4E和4E-BP1在宫颈癌组中的阳性表达显著高于正常组和CIN组（P＜0.01）,而后两组比较差异无统计学意义（P＞0.05）;（2）eIF4E表达与宫颈癌临床分期、淋巴结转移、病理分化程度和术后复发有关（P＜0.01,P＜0.01,P＜0.05,P＜0.05）;（3）mTOR表达与宫颈癌术后复发显著相关（P＜0.01）;（4）4E-BP1表达与宫颈癌临床分期显著相关（P＜0.01）;（5）mTOR与eIF4E;mTOR与4E-BP1在宫颈癌中的阳性表达均呈显著正相关（P＜0.01）;（6）生存单因素分析mTOR、eIF4E、4E-BP1均与宫颈癌的预后有关（P＜0.01,P＜0.05,P＜0.05）.结论 mTOR、eIF4E、4E-BP1的高表达在宫颈癌发生发展中起重要作用,与预后不良有关.

Objective To investigate the effects of the expression of mTOR,eIF4E and 4E - BP1 in the development and advancement of cervix carcinoma, in order to provide some valuable evidences for prognosis. Methods The expression of mTOR,eIF4E and 4E - BP1 in tumor tissues of 53 patients with cervix carcinoma and 15 cases with cervical intraepithelial neoplasia（CIN） and 13 cases with normal cervical epithelium were detected by S - P immunohistochemieal technique. Results （ 1 ） The expression of mTOR,eIF4E and 4E - BP1 in tumor tissues were higher than those in CIN and normal cervical epithelial tissues （P 〈 0.01 ） , and there was no significant difference in mTOR、eIF4E and 4E - BP1 staining between normal cervical epithelium and CIN groups （ P 〉 0.05 ）. （ 2 ） eIF4E positive - expression correlated significantly with disease stage ,metastasis of pelvic lymph node （ P 〈 0.01 ）, tumor cell differentiation and recurrence （P 〈 0.05 ）. （3） mTOR positive - expression was related to recurrence （ P 〈 0.01 ）. （ 4 ） 4E - BP1 positive - expression correlated significantly with disease stage （ P 〈 0.01 ）. （ 5 ） mTOR positive - expression correlated significantly with elF4E and 4E - BP1 （ P 〈 0.01 ）. （ 6 ） The analysis of single factor revealed that the expression of mTOR,eIF4E and 4E - BP1 was significantly related with prognosis of patients（P 〈0.01 ,P 〈 0.05 ,P 〈 0.05 ）. Conclusion The increased expression of mTOR,eIF4E and 4E -BP1 may play an important role in the development and progression of the cervical carcinoma,which is associated with poor prognosis.