五种方法检测抗dsDNA抗体性能比较与临床评价

Performances of Five Methods for the Detection of Anti-dsDNA Antibody

用化学发光免疫分析法(CLIA)、免疫印迹法(WB)、间接免疫荧光法(IIF)、胶体金免疫层析法(CGCIA)和ELISA检测dsDNA抗体,评价方法的分析性能、相关性及对系统性红斑狼疮(SLE)的诊断价值。用五种方法检测300例SLE组患者、300例疾病对照组患者和100名健康人对照组血清抗dsDNA抗体。经统计学分析,CLIA法灵敏度最高,其次是ELISA、CGCIA、WB、IIF。IIF法特异性最高。CLIA法与ELISA法、WB法与CLIA法之间相关性χ2检验无显著性差异(P>0.05)。CLIA法与ELISA法高度一致(Kappa值0.909)。本研究提示ELISA与IIF法联合是临床实验室检测dsDNA抗体的最佳方案。

To evaluate the performances of five methods for the detection of anti-dsDNA antibody.The serum anti-dsDNA antibody levels in 300 systemic lupus erythematosus（SLE） patients,300 disease control patients and 100 healthy peoples were detected by chemiluminescence immunoassay（CLIA）,western blotting（WB）,indirect immuno-fluorescence（IIF）,enzyme-linked immuno-sorbent assay（ELISA） and colloidal gold colloid immunochromatography assay（CGCIA） respectively.The results showed that the highest sensitivity of assay was CLIA,followed by ELISA,CGCIA,WB and IIF.The highest specificity of Assay was IIF.There was no differences in the assay results between ELISA and CLIA,WB and CLIA（P0.05）.The results measured by ELISA and CLIA matched very well with clinical diagnosis verification with high coincidence（Kappa=0.909）.The results indicate that it may be the best way to measure anti-dsDNA antibody by ELISA and IIF simultaneously in clinical laboratory.