中国汉族人群通用血浆制备方法研究

Study on preparation of universal plasma in Chinese Han population

目的探讨我国人群通用血浆制备的最佳配比方案。方法用试管凝集法确定A、B、AB型血浆两两之间的最佳比例，然后计算出三种血型的搭配比例，以血浆比例、时间、温度3个因素进行正交设计。观察3个因素对混合血浆抗体效价的影响。结果三种血型血浆两两之间的最佳比例分别是A：B=3：1、B：AB=1：3、A：AB=1：2；正交试验结果表明将A：B的比例定位于3：1附近，再按照B：AB=1：3、A：AB=1：2去补偿，抗体效价可降到通用血浆的标准，且时间、温度对血浆抗体效价无影响。结论实验结果表明，A、B、AB三种血型血浆按照接近中国人血型比例6：2．5：1混合，于22℃孵育1h可以得到抗血型抗体IgM效价符合标准的混合血浆。

Objective To explore optimal preparation method of universal plasma in Chinese Han population. Methods The optimal plasma ratios of A：B, A：AB and B：AB were assayed by tube agglutination method. Three factors influencing the preparation of universal plasma such as the proportion of plasma, reaction temperature and time, were analyzed by orthogonal design. Results The optimal plasma ratios were A：B= 3：1, B：AB= 1：3, and A：AB= 1：2 respectively among 3 blood group. Orthogonal design showed antibody titers might be reduced to the standard of universal plasma with the plasma ratio of A：B= 3：1, B：AB= 1：3 and A：AB= 1：2. However, the reaction temperature and time did not affect the antibody titers significantly. Conclusion Re- search data showed that the optimal universal plasma in Chinese Han population might be prepared by pooling plasma A：B：AB= 6：2.5：1 at 22℃ for 1 h.