君子兰组织培养研究

Study on Tissue Culture of Clivia miniata Regel.

[目的]筛选适宜君子兰叶片离体的条件。[方法]选取君子兰(Clivia miniata Regel)品种"油匠"的叶片为外植体进行离体培养,在MS培养基中附加不同浓度的2,4-D、BA、NAA和KT,对外植体采用不用时间的低温预处理,并对接种后的外植体分别进行不同时长的暗培养,研究不同培养条件对外植体愈伤组织诱导和分化的影响。[结果]叶片诱导分化的最佳培养基为MS+BA2.0 mg/L+NAA3.0 mg/L+KT1.0 mg/L+蔗糖3.0%,诱导与分化率分别为59.2%和55.2%;暗培养10d后外植体诱导与分化率较高,为13.8%和25.0%。[结论]培养基中加入活性炭对叶片诱导与分化不利;接种前低温预处理1 d,叶片诱导与分化率较高,分别为33.3%和25.0%。

[Objective] Selected the best medium for vitro cuhure. [Method] The leaf of Clivia miniata Regel. ＇Youjiang＇ was used as explants for in vitro culture. The explants were cultured on MS medium supplemented with different concentration of 2,4-D,BA,NAA,KT and were treated with low temperature pretreatment. The inoculation explants were treated with dark culture. The influence of callus induction and differentiation were studied. [Result] The results showed that the best optimum medium was MS＋BA2.0 mg/L＋NAA3.0 mg/L＋KT1.0 mg/L sucrose with induction and differentiation rates of 59.2 and 55.2 percentages; The induction rate were higher is 13.8 and 25.0 percentages after clark culturing for 10 day, [Conelusionl The active carbon was harmful to the callus induction and differentiation of leaf because of enhancing the browning of explants. The rates of induction and differentiation were higher after dark culturing forld and treating in 4 ℃ before inoculation with rates of 33.3 and 25.0 percentages respectively.