磁性纳米复合粒子富集胎儿细胞在无创性产前诊断22q11.2微缺失综合征中的应用

Enrichment of fetal cells by magnetic nanoparticle in the application of non-invasive prenatal diagnosis 22q11.2 microdeletion syndrome

目的探讨利用磁性纳米复合粒子富集胎儿细胞进行无创性产前诊断22q11.2微缺失综合征的可行性。方法收集研究对象26例,分别经过磁性纳米粒子富集胎儿有核红细胞(研究组)及羊水穿刺胎儿细胞(对照组)进行荧光原位杂交(FISH)检测确定22q11.2是否存在异常,比较两组之间存在的差异。结果两组在诊断22q11.2微缺失综合征方面一致率为100%(2/2),证实了利用该方法实现无创性产前诊断22q11.2微缺失综合征的可行性。结论磁性纳米复合粒子富集胎儿细胞诊断22q11.2微缺失综合征为无创性的产前诊断,检测过程快速、准确、高效,较羊水穿刺胎儿细胞进行荧光原位杂交法具有无创及省力等优点,可望用于产前诊断22q11.2微缺失综合征,是一种值得推广的方法。

Objective Exploring the feasibility of using magnetic nanocomposite particles fetal cells enrichment to noninvasive prenatal diagnosis 22q11.2 microdeletion syndrome.Methods Collecting 26 patients as research object,and using magnetic nanoparticles enrichment fetal nucleated red blood cells（the team） and amniotic fluid puncture fetal cells（control group） on fluorescence in situ hybridization（FISH） to check respectively for making sure whether there are 22 q11.2 abnormal,and comparing the differences between the two groups.Results Two groups in the aspect of prenatal diagnosis 22q11.2 microdeletion syndrome consistent rate was 100%（2/2）.It can confirmed that the use of this non-invasive prenatal diagnosis of 22q11.2 microdeletion syndrome is feasible.Conclusion Using magnetic nanoparticle to enrich fetal cells for non-invasive prenatal diagnosis 22q11.2 microdeletion syndrome,and comparing amniotic fluid puncture for cells,the testing process has a lot of advantages such as rapid,accurate,efficient,non-invasive and energy.It is a worthy expansion of method of prenatal diagnosis 22q11.2 microdeletion syndrome.