反相高效液相色谱法测定升麻药材中升麻苷H-1的含量

Determination of Cimicifugoside H-1 in Rhizoma Cimicifugae by RP-HPLC

建立升麻药材中升麻苷H-1含量测定的反相高效液相色谱分析方法。使用H&E XP ODS-A色谱柱(4.6 mm×250 mm5,μm),流动相为乙腈-水-磷酸(35∶65∶0.4),流速为1 mL/min,柱温为26℃,检测波长203nm。测得升麻苷H-1线性范围为0.95～28.5μg/mL(r=0.9999),平均回收率(n=6)为99.35%(RSD=2.79%),不同来源批次升麻药材含量测定结果表明,升麻药材中升麻苷H-1含量为0.53%～1.09%,综合分析批样品含量测定结果,建议升麻药材中含升麻苷H-1应不低于0.4%。本方法简便、准确、重复性好、专属性强,可作为升麻药材质量控制方法。

To establish an RP-HPLC method for determination of cimicifugoside H-1 in Rhizoma Cimicifugae.HE XP ODS-A（4.6×250 mm,5 μm） column was employed with acetonitrile-water-phosphoric acid（35∶ 65∶ 0.4） as mobile phase,the flow rate was 1.0 mL/min.The column temperature was set 26 ℃ and detection wavelength was set at 203 nm.The linear range of cimicifugoside H-1 was 0.95~28.5 μg,the correlation coefficient was 0.9999 and the average recoveries（n=6） was 99.35%.The method is simple,rapid and reliable,which can be regarded as a reasonable method for determining the content of cimicifugoside H-1 in Rhizoma Cimicifugae.The results can be supported as data bases for developing quality specification of Rhizoma Cimicifugae.