摘要
本文采用正交实验优化提取方法,HPLC法测定五味子醇甲含量,对五味子的快速提取工艺方法进行了研究。结果表明:以80%乙醇作为提取液,电场强度10 kv/cm,脉冲数为10,料液比1∶10为最佳提取工艺;影响因素的大小依次为:电场强度、料液比、脉冲数。此法提取五味子中的五味子醇甲,提取率可达0.76%,得膏率达27.886%。本提取工艺可行,经济、省时、回收率高。
The rapid extracting technology for schisandrol A from Schisandra chinensis was investigated by using orthogonal experimental design,and the content of schisandrol A was detected by HPLC.The optimum extracting technology was obtained as follows: 80% ethanol as for the extract liquid,10 kv/cm electric field intensity,impulsion frequency of 10,and 1∶ 10 as the ratio for material and liquid.The effect levels of these factors successively were electric field intensity,the ratio for material and liquid,and impulsion frequency.The content of schisandrol A was 0.76% in S.chinensis,and the yield of extract was 27.886%.The extracting technology is feasible,economic with high recovery ratio and saving time in the process.
出处
《天然产物研究与开发》
CAS
CSCD
2011年第4期755-757,共3页
Natural Product Research and Development
基金
科技部科技支撑项目(2007GAI38B03
2006BAI06A14-03)
关键词
北五味子
五味子醇甲
提取工艺
正交实验
高压脉冲电场
Schisandra chinensis
schisandrol A
extracting technology
orthogonal experiment
high voltage pulsed electric field