多重危险因素致小鼠动脉粥样硬化协同作用

Influence of multiple risk factors on atherosclerosis in mice

目的探讨多重危险因素对小鼠动脉粥样硬化发生发展的影响。方法小鼠随机分为6组:空白对照组(A)、高脂组(B)、普食+CPn组(C)、高脂+CPn组(D)、普食+Cpn+蛋氨酸组(E)、高脂+CPn+蛋氨酸组(F),分别饲以相应饲料或接种CPn;采血检测血脂、取胸主动脉检测超微结构变化。结果其他各组总胆固醇(TC)、甘油三酯(TG)、血浆动脉硬化指数(AIP)明显高于A组(均P<0.01),内皮细胞和平滑肌细胞不同程度受损伤;D组TC、LDL-C、ox-LDL明显高于B组(P<0.05或P<0.01);F组TC、ox-LDL明显高于B组(P<0.05或P<0.01);C组LDL-C明显高于A组(P<0.05)。结论 CPn感染促使正常饮食小鼠血脂代谢紊乱,加重高脂饮食所致的血脂代谢紊乱;高脂、CPn、蛋氨酸可能协同促进动脉粥样硬化发生。

Objective To explore the influence of multiple risks on the generation and development of atherosclerosis. Methods Mice were randomly divided into 6 groups ： normal （ group A ）, high fat diet （ group B ）, Chlamydia pneumoniae （ CPn ） （ group C）, high fat diet ＋ CPn （ group D）, CPn ＋ methionine （ group F ）, high fat diet ＋ CPn ＋ methionine （ group E）. All the mice were fed with specific diet or inoculated with CPn. The blood of the mice was collected to detect the level of blood fat,and thoracic aorta was taken to observe ultra-structrue changes. Results Compared with group A, the level of total cholesterol （ TC ）, tryglyceride （ TG ）, and atherogenic index of plasma （ AIP ） in other groups was obviously higher （ P 〈0. 01 for all）. And the endothelial cells and smooth muscle cells in all groups except group A were damaged in some extent. Compared with group B, the level of TC, low density lipoprotein-cholesterol （ LDL-C ）, and oxidized low density lipoprotein（ox-LDL） were higher（ P 〈0. 05 or P 〈0.01 ） ,and the level of TC ,ox-LDL in grouo F were higher significantly （ P 〈 0.05 or P 〈 0. 01 ）. The level of LDL-C in group C was obviously higher than that of in group A（ P 〈 0. 05 ）. Conclusion CPn infection promotes the development of dyslipidosis and aggravates dyslipidosis induced by high fat diet. CPn and methionine can promote genesis and development of atherosclerosis in mice.