β-CD-FeC_4Pc包合物模拟酶催化氧化苯酚测定H_2O_2

Determination of hydrogen peroxide based on the oxidation reaction of phenol using a β-cyclodextrin-Iron(Ⅲ)-tetracarboxyphthalocyanine inclusion complex as mimetic peroxidase

研究了四羧基铁酞菁(FeC4Pc)与β-环糊精(β-CD)形成的包合物作为过氧化物模拟酶对H2O2-苯酚-4-氨基安替比林(4-AAP)显色体系的催化特性和反应条件。试验表明,体系在pH 11的Britton-Robinson(B-R)缓冲溶液中于496nm波长处有最大吸收,吸光度与H2O2含量成比例关系,线性范围为7.63×10-6～2.14×10-4 mol/L,由此建立了测定H2 O2的新方法,检出限为9.40×10-7 mol/L。该方法可用于实际样品的测定。

The catalytic activity of β-cyclodextrin-iron（Ⅲ）-tetracarboxyphthalocyanine inclusion complex was studied.Also,catalytic characteristic and reaction conditions of hydrogen peroxide-phenol-4-aminoantipyrine system were investigated.In pH 11 Britton-Robinson buffer solution,products of this system exhibited maximum absorption at 496 nm,and the absorbance of the products was proportional to the amount of H2O2.The linear range was 7.63×10-6~ 2.14×10-4 mol/L.Based on this,a new spectrophotometric method for the determination of H2O2 was proposed.The detection limit was 9.40×10-7 mol/L.The proposed method was applied to the analysis of real samples with satisfactory results.