HPLC指纹图谱结合系统聚类分析法对灵芝孢子油质量的研究

Study of the Quality of Ganoderma lucidum Spore Oil by HPLC Fingerprints and Genealogzied Cluster Analysis

目的:建立灵芝孢子油的高效液相色谱指纹图谱,并结合聚类分析法初步考察不同采收时间、产地及破壁工艺对灵芝孢子油质量的影响。方法:采用反相高效液相色谱法测定灵芝孢子油中甘油三油酸酯的含量,色谱柱为LiChrospher RP-18e(250mm×4.6mm,5μm),流动相为甲醇-异丙醇(梯度洗脱),蒸发光散射检测器检测。采用系统聚类法对灵芝孢子油样品色谱中各特征峰的相对峰面积进行分析。结果:系统聚类法将供试品分成两大类,其中采收时间及破壁工艺对灵芝孢子油的质量影响明显,产地影响则不明显。结论:该方法能较好地为灵芝孢子油的质量评估提供有益的信息。

OBJECTIVE： To establish the HPLC fingerprints of Ganoderma lucidum spore oil, and to study the impact of producing area, collection time and wall broken technology using Genealogzied clustering analysis. METHODS： RP-HPLC was used to determine the content of glycerin trioleate in G. lucidum spore oil. The determination was performed on LiChrospher RP-18e colunto（250 mm×4.6 mm, 5 μm）with mobile phase consisted of methanol-isopropanol （gradient elution） using Evaporative Light Scattering Detector （ELSD） ; Genealogized cluster analysis was used to analyze relative peak area of G. lucidum spore oil. RESULTS： Test samples were divided into two categories by hierarchical clustering analysis. The collection time and technique of breaking wall had great impact on the quality of G. lucidum spore oil, while the producing area had not. CONCLUSION： The method can be used to provide some useful information for quality evaluation of G. lucidum spore oil.