摘要
以怀地黄花药为外植体,通过器官发生途径建立怀地黄再生植株体系.结果表明,在4℃条件下预处理3 d有利于愈伤组织的诱导;愈伤组织诱导的最佳培养基为2.0 mg·L-1MS+2,4-D+0.5 mg·L-16-BA+2.0 mg·L-1KT;愈伤组织分化不定芽的最佳培养基为10 mg·L-1MS+IAA+10 mg·L-16-BA.
Taking anthers from Rehmannia glutinosa Libosch as expants,the plantlet regeneration system has been established through organgenesis.The results showed that pretreatment of anther(treated at 4 ℃ for 3 d) has a positive effect on callus induction the optimum medium components for callus induction were found to be 20 mg·L-1MS+2,4-D+10 mg·L-16-BA+0.5 mg·L-1KT,and for callus differentiation was 10 mg·L-1MS+IAA+10 mg·L-16-BA.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2011年第4期383-386,共4页
Journal of Henan Agricultural University
基金
国家高技术研究发展计划项目(2006AA100109)
河南省教育厅自然科学研究计划项目(2009B1800162011B180035)
关键词
怀地黄
花药培养
植株再生
Rehmannia glutinosa Libosch
anther cultivation
plantlet regeneration