半夏疏松愈伤组织诱导及高产半夏蛋白悬浮株系筛选

Loose Callus Induction and Screening of Cell Suspension Lines with High Pinellin from Pinellia ternata(Thunb.) Breit

筛选高产半夏蛋白悬浮株系。从半夏块茎、叶柄、叶片三种植物组织中选出最优外植体,以正交实验分析该外植体组织培养和继代培养最适条件,建立半夏蛋白悬浮株系,采用单因子法筛选高产株系。结果显示:以半夏块茎为外植体诱导愈伤组织效果最好,其愈伤组织诱导最适培养基为:MS+2,4-D 2.0 mg/L+6-BA 1.0 mg/L;其继代增殖最适培养基为:MS+2,4-D 1.0 mg/L+6-BA 1.0 mg/L+NAA 0.05 mg/L;高产半夏蛋白悬浮株系最优培养基为:MS+6-BA 0.4 mg/L+NAA 0.1 mg/L,生长良好且可生产较多半夏蛋白。

Screening of cell suspension lines with high pinellin from Pinellia ternata（Thunb.） Breit Tuber.Screening of the best explant from three plant tissues which are tuber,petiole,leaf of Pinellia ternata.For the best explant,analysising the optimal conditions of tissue culture and subculture by orthogonal experiment.Establishing cell suspension lines with pinellin,and screening of high-yielding strain by single-factor method.The results show that the best explant which induces the callus is the tuber of Pinellia ternata.The best medium of induceing the callus is that： MS＋2,4-D 2.0 mg/L＋6-BA 1.0 mg/L;The best medium of subculture is that： MS＋2,4-D 1.0 mg/L＋6-BA 1.0 mg/L＋NAA 0.05 mg/L;Cell suspension lines with high pinellin is that： MS＋6-BA 0.4 mg/L＋NAA 0.1 mg/L,which can grow well and produce more pinellin.