外源GA_3对白桦成花基因影响的定量PCR分析

Effect of Exogenous GA_3 on Flowering Genes Expression of Betula platyphylla by Real-Time Fluorescent Quantitative PCR

植物开花是高等植物由营养生长向生殖生长转变的重要生理过程,是个体发育和后代繁衍的中心环节。赤霉素是调节植物生长发育的五大激素之一（Takahashi et al.,1991）,赤霉素处理也是人工调控植物成花的最有效途径之一,

This study investigated the effect of gibberellin on flower formation of Betula platyphylla and the possible mechanism in the process. In this experiment, different concentrations of GA_3 （50,100,200 mg·L-1） were sprayed on the leaves of B. platyphylla at the stage of flower induction. The expression of the key genes involved in B. platyphylla flower formation in the apical meristem was studied during the period of flowering differentiation by RT-PCR. The results showed that flowering induction of B. platyphylla was in late May in Harbin and flowering transition was significantly inhibited by different concentrations of GA_3. The expression of flowering-promoted genes, including LFY, GI, ELF3 and LD, were distinct lower than the control group, while expression of the flowering suppressor gene FLC had no significant changes. Therefore, the exogenous GA_3 was of the disincentive during the flowering transition of B. platyphylla. GA_3 plays a negative role in different gene expression of the multiple flowering pathways rather than individual one.