紫外分光光度法测定白千层枝和叶总三萜酸含量

Ultra-violet Spectrophotometric Determination of Triterpenoidic Acid in the Stems and Leaves of Melaleuca leucadendra

以5%香草醛-冰乙酸和高氯酸为显色剂,测定白千层枝和叶中三萜酸的总含量。熊果酸对照品在0.20～1.60 mg/L范围内呈良好线性关系,其回归方程为A=0.1548C+0.0286,r=0.999 3。结果表明白千层枝中三萜酸的总质量分数约为1.096%,叶中三萜酸的总质量分数约为1.017%。

The total content of triterpenoidic acid in the stems and leaves of Melaleuca leucadendra Linn.was determined using 5 % vanillin-acetic acid and perchloric acid as color system.Ursolic acid had a better linear relationship in the range of 0.20-1.60 mg/L and its regression equation was A=0.154 8C＋0.028 6（r=0.999 3）.The results showed that the total mass fractions of triterpenoidic acid in the stems and leaves of M.leucadendra were 1.096 % and 1.017 % respectively.