离子对HPLC法测定五维牛磺酸口服液中4种水溶性维生素

Determination of Four Vitamins in Five Vitamins and Taurine Oral Solution by IP-HPLC

建立HPLC法测定五维牛磺酸口服液中维生素B1、B2、B6和烟酰胺的含量。采用C18柱,甲醇-0.01mol·L-1庚烷磺酸钠溶液(含0.1%冰醋酸)为流动相,检测波长273 nm。平均回收率分别为99.8%、99.7%、99.4%和99.4%。

An HPLC method was established for nicotinamide in Five Vitamins and Taurine Oral liquid. A methanol-0.01 mol·L^-1 heptanesulfonic acid sodium salt wavelength was 273 nm. The average recoveries of vitamin the determination of C18 coltlmn was used, vitamin B1, B2, B6 and with the mobile phase of （contain 0.1% glacial acetic acid）. The detection B1, B2, B6 and nicotinamide were 99.8%, 99.7%,99.4% and 99.4%, respectively.