SVC血清NO变化对精子活动率的影响

目的探讨亚临床型精索静脉曲张(SVC)患者精索静脉血清一氧化氮(NO)含量与精子数量和活动率的关系。方法选择亚临床型精索静脉曲张(SVC)患者16例做为试验组(n=16),选择正常生育能力患者16例做为对照组(n=16)。采用硝酸还原酶比色法测定两组左侧精索静脉及外周静脉血清NO的含量。应用计算机辅助精液分析技术监测试验组各项精液指标。结果 1.试验组左侧精索静脉血清NO含量为(120.90±10.60)μmol/L,外周静脉血清NO含量为(84.26±12.65)μmol/L。对照组左侧精索静脉血清内NO含量为(78.33±13.37)μmol/L,外周静脉血清NO含量为(74.17±15.32)μmol/L。试验组左侧精索静脉血清NO含量高于对照组。两者比较差异具有统计学意义(P<0.05)。2.试验组左侧精索静脉血清NO水平与精子活动率水平呈负的直线相关性,具有统计学意义(P<0.05)。精子密度与左侧精索静脉血清NO水平不呈直线相关性。结论亚临床型精索静脉曲张患者左侧精索静脉血清NO含量增高,并与精子活动率呈负相关,但与精子密度不呈直线相关性。

Objective To investigate the effect of nitric oxide content in serum on sperm motility in sub-clinical varicocele.Methods Sixteen infertile men with sub-clinical varicocele were enrolled as study group（n=16） and sixteen normal fertile men with inguinal hernia were enrolled as control group（n=16）.The NO content in the left spermatic vein and peripheral vein serum of the tow groups were measured by nitricacid reductase method,and the semen parameters of SVC were determined by computer assisted semen analysis（CASA）.Results 1.In study group the mean NO concent in left spermatic vein serum is 120.90±10.60umol/L.the mean NO concent in peripheral vein blood serum is 84.26±12.65umol/L.In control group,the mean NO concent in left spermatic vein blood serum is 78.33±13.37umol/L.The NO concent in peripheral vein serum is 74.17±15.32umol/L.NO concent in the left spermatic vein blood serum of study group are higher than that of control group.Difference was significant（P〈0.05）.2.Linear correlation analysis indicated that the NO content in the left spermatic vein serum of study group were significantly negatively associated with the sperm motility rate（P〈0.05）.However,the sperm density and the NO content in the left spermatic vein blood serum were not linear correlation.Conclusion The NO content in left spermatic vein blood serum sub-clinical has increased,and it is negatively correlated with the rate of sperm activity but it is not linearly correlated with the sperm density.