摘要
目的:探讨细胞外液不同的钠浓度对培养新生小鼠心肌细胞Na+电流的影响。方法:采用膜片钳技术,在浴液不同的钠离子浓度下,记录培养新生小鼠的心肌细胞钠电流的激活、失活以及恢复曲线。结果:相对于低钠浴液,高钠浴液记录的培养新生小鼠心肌细胞钠电流明显延迟,电流幅度增加也不明显,激活曲线不圆滑。结论:胞外高钠不是记录钠电流最合适的实验方案。
Objective： To study the effect of different extracellular sodium concentration on Na＋ current in cultured cardiomyocytes of suckling mouse.Methods： Using the patch clamp technique of the whole cell configuration,with the different concentration of sodium in bath solution,Na＋ current of activation,inactivation and recovery curve were recorded.Results：Compared with low sodium bath solution,Na＋ current was significantly delayed,the increase of current was not obvious,and Ⅰ-Ⅴ curve was not smooth in high sodium bath solution.Conclusion： Extracellular solution with high sodium is not appropriate to record Na＋ current..
出处
《泸州医学院学报》
2011年第4期341-344,共4页
Journal of Luzhou Medical College
关键词
钠电流
膜片钳
心肌细胞
Sodium current
Patch clamp
Cardiomyocyte
