骨髓间充质干细胞复合壳聚糖凝胶体外构建可注射组织工程软骨

IN VIRTO CONSTRUCTION OF INJECTABLE TISSUE-ENGINEERING CARTILAGE WITH MESENCHYMAL STEM CELLS AND CHITOSAN GELS

目的:探讨壳聚糖/β-甘油磷酸钠凝胶用作骨髓间充质干细胞的载体体外构建可注射型组织工程软骨的有效性和可行性。方法:骨髓间充质干细胞与壳聚糖/β-甘油磷酸钠制成注射型细胞/凝胶复合物并行体外成软骨诱导培养,通过倒置显微镜、扫描电镜和组织学检查等观察细胞在凝胶内黏附、生长、增殖、向软骨细胞分化及形成软骨样组织等情况。结果:在体外成软骨培养条件下,壳聚糖凝胶支持骨髓间充质干细胞生长、增殖、向软骨细胞分化并形成软骨样组织。结论:骨髓间充质干细胞/壳聚糖/β-甘油磷酸钠凝胶复合物,有望用作可注射型组织工程软骨修复软骨缺损。

Objective： To explore the effectiveness and the feasibility of chitosan/β-glycerophosphate disodium（C/GP） gels as carriers for mesenchymal stem cells（MSCs） to construct injectable tissue-engineering cartilage in vitro.Methods： BMSCs were incorporated into C/GP mixture to construct injectable BMSCs-C/GP gel complex,then chondrogenesis was induced-cultured in vitro.The adhesion,growth,proliferation and chondrogenic differentiation of BMSCs into C/GP gel and chondrogenesis of BMSCs-C/GP gel complex were estimated by inverted phase contrast microscope,scanning electron microscope and histology.Results： C/GP gel supported the growth,proliferation and chondrogenic differentiation of BMSCs,and BMSCs-C/GP gel complex could form cartilage-like tissue when chondrogenesis was induced-cultured in vitro.Conclusion： BMSCs-C/GP gel complex can be used as injectable tissue-engineering cartilage to repair cartilage defects.