摘要
A short ionic liquids （ILs）-based monolithic cartridge was prepared and used as the selective extraction sorbent. After the material was evaluated by field emission-scanning electron microscopy （FE-SEM）, a new approach for the extraction and determination of quercetin and myricetin from Chamaecyparis obtusa （C. obtusa） by using ILs-based, monolithic cartridge system was developed. Chromatographic analysis was conducted on a C18 column with UV detection at 372 nm, an eluting solution consisting of acetonitrile-water （25/75, V/V） as the mobile phase, and a flow rate of 0.7 mLomin 1. A good linear relationship was demonstrated when the concentrations of quercetin and myricetin were in the range of 0.5--100.0 ggomL-1. The recoveries ranged from 101.6% to 104.6% and the inter- and intra-day relative standard deviations （RSD） were less than 5.0%. This method effectively removed the impurities and avoided tedious pretreatment. It provided a fast, economic and effective method for assaying trace drugs from natural plants.
A short ionic liquids （ILs）-based monolithic cartridge was prepared and used as the selective extraction sorbent. After the material was evaluated by field emission-scanning electron microscopy （FE-SEM）, a new approach for the extraction and determination of quercetin and myricetin from Chamaecyparis obtusa （C. obtusa） by using ILs-based, monolithic cartridge system was developed. Chromatographic analysis was conducted on a C18 column with UV detection at 372 nm, an eluting solution consisting of acetonitrile-water （25/75, V/V） as the mobile phase, and a flow rate of 0.7 mLomin 1. A good linear relationship was demonstrated when the concentrations of quercetin and myricetin were in the range of 0.5--100.0 ggomL-1. The recoveries ranged from 101.6% to 104.6% and the inter- and intra-day relative standard deviations （RSD） were less than 5.0%. This method effectively removed the impurities and avoided tedious pretreatment. It provided a fast, economic and effective method for assaying trace drugs from natural plants.
